Experiment set10S257 for Phocaeicola vulgatus CL09T03C04

Compare to:

L-Glutamine nitrogen source; Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen

Group: nitrogen source
Media: Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen + L-Glutamine (10 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 15 uM Iron (II) sulfate heptahydrate, 3 mM Dithiothreitol, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, 3 mM Sodium sulfide nonahydrate, 0.1 ng/L Cyanocobalamin, Mineral 3B solution minus Nitrogen (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 37 genes in this experiment

For nitrogen source L-Glutamine in Phocaeicola vulgatus CL09T03C04

For nitrogen source L-Glutamine across organisms

SEED Subsystems

Subsystem #Specific
LOS core oligosaccharide biosynthesis 2
Folate Biosynthesis 1
KDO2-Lipid A biosynthesis 1
Lactose utilization 1
Maltose and Maltodextrin Utilization 1
Methionine Biosynthesis 1
N-linked Glycosylation in Bacteria 1
Oxidative stress 1
Phosphate metabolism 1
Teichoic and lipoteichoic acids biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
diethylphosphate degradation 2 1 1
Arg/N-end rule pathway (eukaryotic) 14 6 2
poly(glycerol phosphate) wall teichoic acid biosynthesis 11 2 1