Experiment set10S254 for Phocaeicola vulgatus CL09T03C04
L-Serine nitrogen source; Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Group: nitrogen sourceMedia: Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen + L-Serine (10 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 15 uM Iron (II) sulfate heptahydrate, 3 mM Dithiothreitol, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, 3 mM Sodium sulfide nonahydrate, 0.1 ng/L Cyanocobalamin, Mineral 3B solution minus Nitrogen (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 13 genes in this experiment
For nitrogen source L-Serine in Phocaeicola vulgatus CL09T03C04
For nitrogen source L-Serine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Coenzyme B12 biosynthesis | 1 |
| Methionine Biosynthesis | 1 |
| Potassium homeostasis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes: