Experiment set10S249 for Phocaeicola vulgatus CL09T03C04

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L-Asparagine nitrogen source; Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen

Group: nitrogen source
Media: Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen + L-Asparagine (10 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 3 mM Dithiothreitol, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, 3 mM Sodium sulfide nonahydrate, Mineral 3B solution minus Nitrogen (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 1 genes in this experiment

For nitrogen source L-Asparagine in Phocaeicola vulgatus CL09T03C04

For nitrogen source L-Asparagine across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.