Experiment set10IT094 for Burkholderia phytofirmans PsJN

Compare to:

Malonic acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Malonic acid (10 mM)
Culturing: BFirm_ML3_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 1.2 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 4 genes in this experiment

For carbon source Malonic acid in Burkholderia phytofirmans PsJN

For carbon source Malonic acid across organisms

SEED Subsystems

Subsystem #Specific
Malonate decarboxylase 2
Hydantoin metabolism 1
Ketoisovalerate oxidoreductase 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate and ATP formation from acetyl-CoA III 1 1 1
acetate conversion to acetyl-CoA 1 1 1
superpathway of acetate utilization and formation 3 3 1
fatty acid biosynthesis initiation (type II) 3 3 1
ethanol degradation II 3 3 1
ethanol degradation IV 3 3 1
ethanol degradation III 3 2 1
malonate degradation I (biotin-independent) 3 2 1
L-isoleucine biosynthesis V 3 2 1
fatty acid biosynthesis initiation (mitochondria) 4 2 1
chitin deacetylation 4 2 1
malonate degradation II (biotin-dependent) 4 1 1
fatty acid biosynthesis initiation (plant mitochondria) 4 1 1
2-methylcitrate cycle I 5 5 1
superpathway of fatty acid biosynthesis initiation 5 4 1
2-methylcitrate cycle II 6 5 1
L-isoleucine biosynthesis IV 6 4 1
β-alanine biosynthesis II 6 4 1
superpathway of bitter acids biosynthesis 18 3 3
colupulone and cohumulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
lupulone and humulone biosynthesis 6 1 1
pederin biosynthesis 14 2 2
reductive glycine pathway of autotrophic CO2 fixation 9 4 1
cis-geranyl-CoA degradation 9 2 1
bryostatin biosynthesis 19 2 2
superpathway of coenzyme A biosynthesis II (plants) 10 8 1
mycobactin biosynthesis 11 3 1
superpathway of fatty acid biosynthesis I (E. coli) 16 14 1
mupirocin biosynthesis 26 1 1
corallopyronin A biosynthesis 30 2 1
superpathway of fatty acid biosynthesis II (plant) 43 38 1
superpathway of fatty acids biosynthesis (E. coli) 53 50 1