Experiment set10IT092 for Herbaspirillum seropedicae SmR1

Dictyostellum sp.mixed culture-2

Group: micoeukaryotes
Media: LB + Pelleted after growth overnight in LB+Kan50 + CaCl2, MgSO4 (10mM ), pH=7
Culturing: HerbieS_ML4a, 48-well_plate, Aerobic, at 30 (C), shaken=700 rpm
By: VM on 6/23/21
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 8 genes in this experiment

For micoeukaryotes Pelleted after growth overnight in LB+Kan50 in Herbaspirillum seropedicae SmR1

For micoeukaryotes Pelleted after growth overnight in LB+Kan50 across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial hemoglobins	2
Bacterial Chemotaxis	1
Flagellar motility	1
Proteasome bacterial	1
Proteolysis in bacteria, ATP-dependent	1
TCA Cycle	1
Two-component regulatory systems in Campylobacter	1
cAMP signaling in bacteria	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Pyruvate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
partial TCA cycle (obligate autotrophs)	8	7	1
TCA cycle VI (Helicobacter)	9	7	1
TCA cycle VII (acetate-producers)	9	7	1
TCA cycle I (prokaryotic)	10	8	1
superpathway of glyoxylate bypass and TCA	12	10	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	22	1