Experiment set10IT091 for Bacteroides thetaiotaomicron VPI-5482
Varel_Bryant_medium_Glucose with Anhydrotetracycline hydrochloride 0.0125 mM
Group: stressMedia: Varel_Bryant_medium_Glucose + Anhydrotetracycline hydrochloride (0.0125 mM) + Dimethyl Sulfoxide (0.125 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 3 genes in this experiment
For stress Anhydrotetracycline hydrochloride in Bacteroides thetaiotaomicron VPI-5482
For stress Anhydrotetracycline hydrochloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Methylglyoxal Metabolism | 1 |
| RNA processing and degradation, bacterial | 1 |
| Two cell division clusters relating to chromosome partitioning | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes: