Experiment set10IT087 for Marinobacter adhaerens HP15

Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15

Group: mixed community
Media: Pro99_seawater + Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 + Light intensity (20 μE)
Culturing: Marino_ML2_MIT, bottle_100mL, Aerobic, at 24 (C), shaken=0 rpm
By: Sean Kearney on 1-Mar-20
Media components: 50 uM Sodium phosphate monobasic monohydrate, 800 uM Ammonium chloride, 1.17 uM EDTA (disodium salt), 1.18 uM Ferric chloride, 0.008 uM Zinc sulfate heptahydrate, 0.005 uM Cobalt chloride hexahydrate, 0.09 uM Manganese (II) chloride tetrahydrate, 0.003 uM Sodium Molybdate Dihydrate, 0.01 uM Sodium selenite pentahydrate, 0.01 uM Nickel (II) chloride hexahydrate, 1 X Filtered Seawater

Specific Phenotypes

For 5 genes in this experiment

For mixed community Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 in Marinobacter adhaerens HP15

For mixed community Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 across organisms

SEED Subsystems

Subsystem	#Specific
Glycolate, glyoxylate interconversions	3
Photorespiration (oxidative C2 cycle)	3
Cysteine Biosynthesis	1
DNA-binding regulatory proteins, strays	1
Oxidative stress	1
Thioredoxin-disulfide reductase	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glyoxylate and dicarboxylate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycolate and glyoxylate degradation II	2	2	1
glycolate and glyoxylate degradation III	3	1	1
glycolate and glyoxylate degradation I	4	2	1
superpathway of glycol metabolism and degradation	7	4	1
photorespiration III	9	6	1
photorespiration I	9	6	1
photorespiration II	10	6	1