Experiment set10IT085 for Pseudomonas syringae pv. syringae B728a

KB with Anisodamine Hydrobromide () 10 mM

Group: stress
Media: KB + Anisodamine Hydrobromide () (10 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 8/5/19
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 3 genes in this experiment

For stress Anisodamine Hydrobromide () in Pseudomonas syringae pv. syringae B728a

For stress Anisodamine Hydrobromide () across organisms

SEED Subsystems

Subsystem	#Specific
Ammonia assimilation	1
Bacterial Chemotaxis	1
Flagellar motility	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Two-component regulatory systems in Campylobacter	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• Nitrogen metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate biosynthesis I	2	2	2
L-glutamine degradation II	1	1	1
L-glutamine degradation I	1	1	1
ammonia assimilation cycle III	3	3	2
L-glutamate and L-glutamine biosynthesis	7	5	2
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
L-citrulline biosynthesis	8	7	1
superpathway of L-citrulline metabolism	12	9	1