Experiment set10IT085 for Bacteroides thetaiotaomicron VPI-5482

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Varel_Bryant_medium_Glucose with 8-hydroxy-nitroquinoline 0.05 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + 8-hydroxy-nitroquinoline (0.05 mM) + Dimethyl Sulfoxide (0.5 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 5 genes in this experiment

For stress 8-hydroxy-nitroquinoline in Bacteroides thetaiotaomicron VPI-5482

For stress 8-hydroxy-nitroquinoline across organisms

SEED Subsystems

Subsystem #Specific
Butanol Biosynthesis 1
Fermentations: Mixed acid 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
reductive monocarboxylic acid cycle 2 2 1
pyruvate fermentation to acetate IV 3 3 1
pyruvate fermentation to ethanol I 3 2 1
L-threonine degradation I 6 5 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 7 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 10 1
mixed acid fermentation 16 12 1
superpathway of L-threonine metabolism 18 13 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of N-acetylneuraminate degradation 22 16 1