Experiment set10IT080 for Klebsiella michiganensis M5al

Compare to:

RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x

Group: supernatant
Media: RCH2_defined_noCarbon (0.33x) + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.67 x)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 5/2/18
Media components: 0.0825 g/L Ammonium chloride, 0.033 g/L Potassium Chloride, 0.198 g/L Sodium phosphate monobasic monohydrate, 9.9 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.0099 g/L Magnesium Sulfate Heptahydrate, 0.00495 g/L Nitrilotriacetic acid, 0.0033 g/L Sodium Chloride, 0.00165 g/L Manganese (II) sulfate monohydrate, 0.00033 g/L Cobalt chloride hexahydrate, 0.00033 g/L Zinc sulfate heptahydrate, 0.00033 g/L Calcium chloride dihydrate, 0.00033 g/L Iron (II) sulfate heptahydrate, 8.25e-05 g/L Nickel (II) chloride hexahydrate, 6.6e-05 g/L Aluminum potassium sulfate dodecahydrate, 3.3e-05 g/L Copper (II) sulfate pentahydrate, 3.3e-05 g/L Boric Acid, 3.3e-05 g/L Sodium Molybdate Dihydrate, 0.00099 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.033 mg/L Pyridoxine HCl, 0.0165 mg/L 4-Aminobenzoic acid, 0.0165 mg/L Lipoic acid, 0.0165 mg/L Nicotinic Acid, 0.0165 mg/L Riboflavin, 0.0165 mg/L Thiamine HCl, 0.0165 mg/L calcium pantothenate, 0.0066 mg/L biotin, 0.0066 mg/L Folic Acid, 0.00033 mg/L Cyanocobalamin) (final concentrations)
Growth plate: Plate2b B2

Specific Phenotypes

For 5 genes in this experiment

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted in Klebsiella michiganensis M5al

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem #Specific
Lysine Biosynthesis DAP Pathway 1
Multidrug Resistance Efflux Pumps 1
Threonine and Homoserine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-homoserine biosynthesis 3 3 1
dipicolinate biosynthesis 4 3 1
L-methionine biosynthesis IV 4 2 1
spermidine biosynthesis II 4 2 1
ectoine biosynthesis 5 3 1
superpathway of L-threonine biosynthesis 6 6 1
norspermidine biosynthesis 6 4 1
L-lysine biosynthesis III 7 6 1
L-lysine biosynthesis VI 7 6 1
3-dehydroquinate biosynthesis II (archaea) 7 3 1
cremeomycin biosynthesis 7 2 1
superpathway of L-homoserine and L-methionine biosynthesis 8 8 1
superpathway of polyamine biosynthesis III 8 4 1
grixazone biosynthesis 8 2 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 9 1
L-lysine biosynthesis I 9 9 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 9 1
L-lysine biosynthesis II 9 6 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 10 1
chorismate biosynthesis II (archaea) 12 8 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 18 1
aspartate superpathway 25 24 1
platensimycin biosynthesis 26 6 1