Experiment set10IT079 for Bacteroides thetaiotaomicron VPI-5482
Varel_Bryant_medium_Glucose with Zinc Pyrithione 0.025 mM
Group: stressMedia: Varel_Bryant_medium_Glucose + Zinc Pyrithione (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 3 genes in this experiment
For stress Zinc Pyrithione in Bacteroides thetaiotaomicron VPI-5482
For stress Zinc Pyrithione across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycine and Serine Utilization | 2 |
| Pyruvate Alanine Serine Interconversions | 1 |
| Serine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-serine degradation | 3 | 3 | 3 |
| L-tryptophan degradation II (via pyruvate) | 3 | 3 | 2 |
| L-cysteine degradation II | 3 | 2 | 2 |
| D-serine degradation | 3 | 2 | 2 |
| glycine betaine degradation III | 7 | 4 | 3 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| glycine betaine degradation I | 8 | 4 | 3 |
| L-serine biosynthesis I | 3 | 3 | 1 |
| glycine degradation | 3 | 2 | 1 |
| L-methionine biosynthesis II | 6 | 3 | 2 |
| superpathway of L-serine and glycine biosynthesis I | 4 | 4 | 1 |
| L-mimosine degradation | 8 | 4 | 2 |
| glutathione-mediated detoxification I | 8 | 2 | 2 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 12 | 2 |
| purine nucleobases degradation II (anaerobic) | 24 | 12 | 3 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 7 | 1 |