Experiment set10IT078 for Klebsiella michiganensis M5al

Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x

Group: supernatant
Media: Fungal_growth_media_mCAFEs_v1_noCarbon + supernatant from Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (1 x)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 5/2/18
Media components: 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate2b A6

Specific Phenotypes

For 5 genes in this experiment

For supernatant Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted in Klebsiella michiganensis M5al

For supernatant Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nitrogen metabolism