Experiment set10IT074 for Bacteroides thetaiotaomicron VPI-5482

Compare to:

Varel_Bryant_medium_Glucose with Cephalothin sodium salt 0.1 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Cephalothin sodium salt (0.1 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 3 genes in this experiment

For stress Cephalothin sodium salt in Bacteroides thetaiotaomicron VPI-5482

For stress Cephalothin sodium salt across organisms

SEED Subsystems

Subsystem #Specific
Purine conversions 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
CMP phosphorylation 2 2 1
guanosine deoxyribonucleotides de novo biosynthesis I 2 2 1
adenosine deoxyribonucleotides de novo biosynthesis I 2 2 1
pyrimidine deoxyribonucleotide phosphorylation 4 3 2
superpathway of guanosine nucleotides de novo biosynthesis I 6 6 2
UTP and CTP de novo biosynthesis 3 3 1
pyrimidine deoxyribonucleotides de novo biosynthesis I 9 8 3
pyrimidine deoxyribonucleotides de novo biosynthesis III 9 7 3
purine deoxyribonucleosides salvage 6 4 2
pyrimidine deoxyribonucleotides de novo biosynthesis IV 7 5 2
guanosine ribonucleotides de novo biosynthesis 4 4 1
superpathway of pyrimidine nucleobases salvage 4 4 1
superpathway of guanosine nucleotides de novo biosynthesis II 8 7 2
pyrimidine deoxyribonucleotides biosynthesis from CTP 8 6 2
adenosine deoxyribonucleotides de novo biosynthesis II 4 3 1
guanosine deoxyribonucleotides de novo biosynthesis II 4 3 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis 18 16 4
superpathway of pyrimidine deoxyribonucleoside salvage 9 7 2
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) 14 10 3
superpathway of adenosine nucleotides de novo biosynthesis I 5 5 1
superpathway of pyrimidine ribonucleosides salvage 10 9 2
dZTP biosynthesis 5 4 1
ppGpp metabolism 6 6 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 3
superpathway of purine nucleotide salvage 14 13 2
superpathway of adenosine nucleotides de novo biosynthesis II 7 6 1
pyrimidine deoxyribonucleotides de novo biosynthesis II 7 3 1
superpathway of purine nucleotides de novo biosynthesis II 26 23 3
superpathway of pyrimidine ribonucleotides de novo biosynthesis 9 8 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 4