Experiment set10IT066 for Marinobacter adhaerens HP15

Mixed culture; Prochlorococcus marinus str. MED4 10:1 starting OD to HP15

Group: mixed community
Media: Pro99_seawater + Mixed culture; Prochlorococcus marinus str. MED4 10:1 starting OD to HP15 + Light intensity (20 μE)
Culturing: Marino_ML2_MIT, bottle_100mL, Aerobic, at 24 (C), shaken=0 rpm
By: Sean Kearney on 1-Mar-20
Media components: 50 uM Sodium phosphate monobasic monohydrate, 800 uM Ammonium chloride, 1.17 uM EDTA (disodium salt), 1.18 uM Ferric chloride, 0.008 uM Zinc sulfate heptahydrate, 0.005 uM Cobalt chloride hexahydrate, 0.09 uM Manganese (II) chloride tetrahydrate, 0.003 uM Sodium Molybdate Dihydrate, 0.01 uM Sodium selenite pentahydrate, 0.01 uM Nickel (II) chloride hexahydrate, 1 X Filtered Seawater

Specific Phenotypes

For 4 genes in this experiment

For mixed community Mixed culture; Prochlorococcus marinus str. MED4 10:1 starting OD to HP15 in Marinobacter adhaerens HP15

For mixed community Mixed culture; Prochlorococcus marinus str. MED4 10:1 starting OD to HP15 across organisms

SEED Subsystems

Subsystem	#Specific
Mannose-sensitive hemagglutinin type 4 pilus	3
Entner-Doudoroff Pathway	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Methylglyoxal Metabolism	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Ascorbate and aldarate metabolism
• Fatty acid metabolism
• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine degradation
• Lysine degradation
• Arginine and proline metabolism
• Histidine metabolism
• Tryptophan metabolism
• beta-Alanine metabolism
• Glycerolipid metabolism
• Pyruvate metabolism
• 1,2-Dichloroethane degradation
• Propanoate metabolism
• 3-Chloroacrylic acid degradation
• Butanoate metabolism
• Limonene and pinene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
putrescine degradation I	2	1	1
putrescine degradation V	2	1	1
ethylene glycol degradation	2	1	1
phenylethylamine degradation I	2	1	1
phenylethylamine degradation II	2	1	1
phenylethanol degradation	2	1	1
ethanol degradation II	3	3	1
ethanol degradation IV	3	3	1
putrescine degradation IV	3	2	1
ethanol degradation III	3	2	1
L-phenylalanine degradation II (anaerobic)	3	2	1
hypotaurine degradation	3	2	1
styrene degradation	3	1	1
histamine degradation	3	1	1
phytol degradation	4	3	1
fatty acid α-oxidation I (plants)	4	2	1
putrescine degradation III	4	1	1
D-arabinose degradation II	4	1	1
L-tryptophan degradation X (mammalian, via tryptamine)	4	1	1
octane oxidation	5	5	1
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
mitochondrial NADPH production (yeast)	5	2	1
dopamine degradation	5	1	1
3-methyl-branched fatty acid α-oxidation	6	3	1
alkane oxidation	6	1	1
noradrenaline and adrenaline degradation	13	4	2
superpathway of glycol metabolism and degradation	7	4	1
serotonin degradation	7	3	1
ceramide degradation by α-oxidation	7	2	1
limonene degradation IV (anaerobic)	7	1	1
superpathway of ornithine degradation	8	5	1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast)	8	4	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
aromatic biogenic amine degradation (bacteria)	8	1	1
Entner-Doudoroff pathway II (non-phosphorylative)	9	5	1
L-phenylalanine degradation IV (mammalian, via side chain)	9	3	1
superpathway of phenylethylamine degradation	11	10	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	5	1
superpathway of L-arginine and L-ornithine degradation	13	7	1
anaerobic aromatic compound degradation (Thauera aromatica)	27	4	1
superpathway of pentose and pentitol degradation	42	4	1