Experiment set10IT058 for Phaeobacter inhibens DSM 17395

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DinoMM minimal media

Group: nutrient
Media: DinoMM_L_lactateCarbon_HighNutrient
Culturing: Phaeo_ML1, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.3 generations
By: Adam on 29-Jun-16
Media components: 20 g/L Sea salts, 0.3 g/L Ammonium Sulfate, 0.1 g/L Potassium phosphate monobasic, 20 mM Sodium L-Lactate, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 20 genes in this experiment

SEED Subsystems

Subsystem #Specific
ABC transporter dipeptide (TC 3.A.1.5.2) 1
Acetyl-CoA fermentation to Butyrate 1
Acid resistance mechanisms 1
Adenosyl nucleosidases 1
Arginine and Ornithine Degradation 1
Deoxyribose and Deoxynucleoside Catabolism 1
Isobutyryl-CoA to Propionyl-CoA Module 1
Isoleucine degradation 1
Polyamine Metabolism 1
Polyhydroxybutyrate metabolism 1
Purine conversions 1
Synechocystis experimental 1
Triacylglycerol metabolism 1
Valine degradation 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
cadaverine biosynthesis 1 1 1
arginine dependent acid resistance 1 1 1
purine deoxyribonucleosides degradation I 4 3 3
benzoyl-CoA biosynthesis 3 3 2
purine ribonucleosides degradation 6 5 4
purine deoxyribonucleosides degradation II 3 2 2
adipate degradation 5 5 3
superpathway of putrescine biosynthesis 4 4 2
guanine and guanosine salvage I 2 2 1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway) 2 2 1
adenine and adenosine salvage I 2 2 1
putrescine biosynthesis I 2 2 1
xanthine and xanthosine salvage 2 2 1
putrescine biosynthesis III 2 2 1
adenine and adenosine salvage III 4 3 2
superpathway of purine deoxyribonucleosides degradation 7 6 3
2-methyl-branched fatty acid β-oxidation 14 10 6
fatty acid β-oxidation I (generic) 7 5 3
oleate β-oxidation 35 30 14
adipate biosynthesis 5 4 2
fatty acid β-oxidation IV (unsaturated, even number) 5 3 2
fatty acid β-oxidation II (plant peroxisome) 5 3 2
superpathway of polyamine biosynthesis I 8 8 3
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 5 4
fatty acid salvage 6 6 2
aminopropylcadaverine biosynthesis 3 3 1
pyrimidine deoxyribonucleosides degradation 3 3 1
valproate β-oxidation 9 6 3
L-isoleucine degradation I 6 4 2
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) 3 2 1
superpathway of guanine and guanosine salvage 3 2 1
putrescine biosynthesis II 3 2 1
methyl ketone biosynthesis (engineered) 6 3 2
propanoate fermentation to 2-methylbutanoate 6 3 2
adenine and adenosine salvage V 3 1 1
benzoyl-CoA degradation I (aerobic) 7 3 2
fatty acid β-oxidation VI (mammalian peroxisome) 7 3 2
purine nucleotides degradation II (aerobic) 11 10 3
guanosine nucleotides degradation III 4 4 1
inosine 5'-phosphate degradation 4 4 1
superpathway of polyamine biosynthesis II 8 6 2
phospholipid remodeling (phosphatidylethanolamine, yeast) 4 2 1
spermidine biosynthesis III 4 2 1
phenylacetate degradation I (aerobic) 9 6 2
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 3 2
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 5 1
adenosine nucleotides degradation II 5 4 1
glutaryl-CoA degradation 5 3 1
3-phenylpropanoate degradation 10 4 2
4-hydroxybenzoate biosynthesis III (plants) 5 2 1
nucleoside and nucleotide degradation (archaea) 10 3 2
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) 5 1 1
bisucaberin biosynthesis 5 1 1
lupanine biosynthesis 5 1 1
desferrioxamine B biosynthesis 5 1 1
desferrioxamine E biosynthesis 5 1 1
pyruvate fermentation to hexanol (engineered) 11 7 2
superpathway of phenylethylamine degradation 11 6 2
superpathway of arginine and polyamine biosynthesis 17 17 3
superpathway of pyrimidine deoxyribonucleosides degradation 6 6 1
pyruvate fermentation to butanol II (engineered) 6 4 1
L-lysine degradation X 6 3 1
6-gingerol analog biosynthesis (engineered) 6 2 1
nucleoside and nucleotide degradation (halobacteria) 6 2 1
fluoroacetate and fluorothreonine biosynthesis 6 1 1
superpathway of L-arginine and L-ornithine degradation 13 7 2
superpathway of purine nucleotide salvage 14 13 2
ureide biosynthesis 7 6 1
superpathway of glyoxylate cycle and fatty acid degradation 14 10 2
pyruvate fermentation to butanoate 7 3 1
L-lysine degradation I 7 2 1
Spodoptera littoralis pheromone biosynthesis 22 3 3
L-valine degradation I 8 5 1
superpathway of ornithine degradation 8 3 1
pyruvate fermentation to butanol I 8 3 1
2-methylpropene degradation 8 2 1
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 5 1
L-glutamate degradation V (via hydroxyglutarate) 10 4 1
methyl tert-butyl ether degradation 10 2 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 5 1
L-glutamate degradation VII (to butanoate) 12 4 1
androstenedione degradation I (aerobic) 25 6 2
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 5 1
platensimycin biosynthesis 26 6 2
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 1
androstenedione degradation II (anaerobic) 27 4 2
superpathway of testosterone and androsterone degradation 28 6 2
superpathway of cholesterol degradation I (cholesterol oxidase) 42 8 3
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 1
L-tryptophan degradation III (eukaryotic) 15 5 1
salinosporamide A biosynthesis 15 3 1
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 9 3
glycerol degradation to butanol 16 9 1
crotonate fermentation (to acetate and cyclohexane carboxylate) 16 4 1
superpathway of hyoscyamine (atropine) and scopolamine biosynthesis 16 4 1
arsenic detoxification (mammals) 17 8 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 7 1
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 4 1
cholesterol degradation to androstenedione I (cholesterol oxidase) 17 2 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 11 1
toluene degradation VI (anaerobic) 18 4 1
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) 22 3 1
superpathway of cholesterol degradation III (oxidase) 49 5 2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 20 1
1-butanol autotrophic biosynthesis (engineered) 27 19 1
superpathway of L-lysine degradation 43 10 1