Experiment set10IT056 for Phaeobacter inhibens DSM 17395

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DinoMM minimal media with L-Threonine

Group: nutrient
Media: DinoMM_L_lactateCarbon_HighNutrient + L-Threonine (1 mM)
Culturing: Phaeo_ML1, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 6.6 generations
By: Adam on 29-Jun-16
Media components: 20 g/L Sea salts, 0.3 g/L Ammonium Sulfate, 0.1 g/L Potassium phosphate monobasic, 20 mM Sodium L-Lactate, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 8 genes in this experiment

For nutrient L-Threonine in Phaeobacter inhibens DSM 17395

For nutrient L-Threonine across organisms

SEED Subsystems

Subsystem #Specific
Arginine Deiminase Pathway 1
Arginine and Ornithine Degradation 1
Glycine Biosynthesis 1
Polyamine Metabolism 1
Pyruvate Alanine Serine Interconversions 1
Synechocystis experimental 1
Threonine degradation 1
Triacylglycerol metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine degradation IV 1 1 1
L-threonine degradation III (to methylglyoxal) 3 2 2
L-threonine degradation II 2 2 1
aminopropanol phosphate biosynthesis II 4 2 2
phospholipid remodeling (phosphatidylethanolamine, yeast) 4 2 1
superpathway of L-threonine metabolism 18 15 2