Experiment set10IT056 for Bacteroides thetaiotaomicron VPI-5482

Varel_Bryant_medium_Glucose with Doxycycline hyclate 0.000048828125 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Doxycycline hyclate (4.88e-05 mM) + Dimethyl Sulfoxide (0.000488281 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 3 genes in this experiment

For stress Doxycycline hyclate in Bacteroides thetaiotaomicron VPI-5482

For stress Doxycycline hyclate across organisms

SEED Subsystems

Subsystem	#Specific
ATP-dependent RNA helicases, bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.