Experiment set10IT055 for Klebsiella michiganensis M5al
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Group: supernatantMedia: RCH2_defined_noCarbon (0.2x) + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.8 x)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 5/2/18
Media components: 0.05 g/L Ammonium chloride, 0.02 g/L Potassium Chloride, 0.12 g/L Sodium phosphate monobasic monohydrate, 6 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.006 g/L Magnesium Sulfate Heptahydrate, 0.003 g/L Nitrilotriacetic acid, 0.002 g/L Sodium Chloride, 0.001 g/L Manganese (II) sulfate monohydrate, 0.0002 g/L Cobalt chloride hexahydrate, 0.0002 g/L Zinc sulfate heptahydrate, 0.0002 g/L Calcium chloride dihydrate, 0.0002 g/L Iron (II) sulfate heptahydrate, 5e-05 g/L Nickel (II) chloride hexahydrate, 4e-05 g/L Aluminum potassium sulfate dodecahydrate, 2e-05 g/L Copper (II) sulfate pentahydrate, 2e-05 g/L Boric Acid, 2e-05 g/L Sodium Molybdate Dihydrate, 0.0006 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.02 mg/L Pyridoxine HCl, 0.01 mg/L 4-Aminobenzoic acid, 0.01 mg/L Lipoic acid, 0.01 mg/L Nicotinic Acid, 0.01 mg/L Riboflavin, 0.01 mg/L Thiamine HCl, 0.01 mg/L calcium pantothenate, 0.004 mg/L biotin, 0.004 mg/L Folic Acid, 0.0002 mg/L Cyanocobalamin) (final concentrations)
Growth plate: Plate2a B1
Specific Phenotypes
For 1 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Purine conversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes: