Experiment set10IT052 for Bacteroides thetaiotaomicron VPI-5482
Varel_Bryant_medium_Glucose with Furaltadone hydrochloride 0.1 mM
Group: stressMedia: Varel_Bryant_medium_Glucose + Furaltadone hydrochloride (0.1 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 3 genes in this experiment
For stress Furaltadone hydrochloride in Bacteroides thetaiotaomicron VPI-5482
For stress Furaltadone hydrochloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycine and Serine Utilization | 1 |
| Serine Biosynthesis | 1 |
| YeiH | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-serine biosynthesis I | 3 | 3 | 1 |
| superpathway of L-serine and glycine biosynthesis I | 4 | 4 | 1 |
| UMP biosynthesis II | 6 | 6 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 7 | 1 |