Experiment set10IT050 for Klebsiella michiganensis M5al
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM
Group: supernatantMedia: Fungal_growth_media_mCAFEs_v1_noCarbon + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.67 x) + D-Glucose (37 mM)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 5/2/18
Media components: 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate2a A2
Specific Phenotypes
For 5 genes in this experiment
SEED Subsystems
Subsystem | #Specific |
---|---|
Glutathione: Non-redox reactions | 1 |
Methylglyoxal Metabolism | 1 |
Multidrug Resistance Efflux Pumps | 1 |
Purine conversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
Pathway | #Steps | #Present | #Specific |
---|---|---|---|
methylglyoxal degradation I | 3 | 2 | 1 |
methylglyoxal degradation VIII | 3 | 2 | 1 |
superpathway of methylglyoxal degradation | 8 | 5 | 1 |