Experiment set10IT033 for Paraburkholderia graminis OAS925

Plant=Brachypodium_distachyon; PlantTreatment=None; Sample=root; GrowthSubstrate=potting_mix; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=19_days

Group: in planta
Media: + Plant=Brachypodium_distachyon; PlantTreatment=None; Sample=root; GrowthSubstrate=potting_mix; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=19_days
Culturing: Burkholderia_OAS925_ML2, pot, at 26 (C), (Solid)
By: Marta Torres on 2-Aug-21

Specific Phenotypes

For 7 genes in this experiment

For in planta Plant=Brachypodium_distachyon; PlantTreatment=None; Sample=root; GrowthSubstrate=potting_mix; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=19_days in Paraburkholderia graminis OAS925

For in planta Plant=Brachypodium_distachyon; PlantTreatment=None; Sample=root; GrowthSubstrate=potting_mix; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=19_days across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	2
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Peptidoglycan Biosynthesis	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Glutamate metabolism
• D-Glutamine and D-glutamate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
xylitol degradation I	2	2	1
ribitol degradation I	2	1	1
L-arabinose degradation II	3	2	1
erythritol degradation II	3	1	1
L-threitol degradation	4	1	1
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)	8	8	1
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)	8	7	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
superpathway of pentose and pentitol degradation	42	21	3
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
peptidoglycan biosynthesis II (staphylococci)	17	12	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	1
phosphinothricin tripeptide biosynthesis	25	6	1
phosalacine biosynthesis	25	6	1