Experiment set10IT028 for Bacteroides thetaiotaomicron VPI-5482
Varel_Bryant_medium_Glucose with Econazole nitrate salt 0.025 mM
Group: stressMedia: Varel_Bryant_medium_Glucose + Econazole nitrate salt (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 9 genes in this experiment
For stress Econazole nitrate salt in Bacteroides thetaiotaomicron VPI-5482
For stress Econazole nitrate salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glutamate dehydrogenases | 1 |
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 1 |
| Queuosine-Archaeosine Biosynthesis | 1 |
| Ton and Tol transport systems | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glutamate metabolism
- Arginine and proline metabolism
- Nitrogen metabolism
- Fatty acid biosynthesis
- Ubiquinone and menaquinone biosynthesis
- alpha-Linolenic acid metabolism
- Benzoate degradation via CoA ligation
- Nicotinate and nicotinamide metabolism
- Limonene and pinene degradation
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamate degradation I | 1 | 1 | 1 |
| L-glutamate biosynthesis III | 1 | 1 | 1 |
| NAD salvage pathway IV (from nicotinamide riboside) | 2 | 1 | 1 |
| L-alanine degradation II (to D-lactate) | 3 | 2 | 1 |
| ethene biosynthesis IV (engineered) | 3 | 1 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 5 | 1 |
| L-glutamate degradation XI (reductive Stickland reaction) | 7 | 2 | 1 |
| 4-aminobutanoate degradation V | 7 | 1 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 2 | 1 |
| superpathway of NAD biosynthesis in eukaryotes | 14 | 6 | 1 |
| methylaspartate cycle | 19 | 11 | 1 |