Experiment set10IT019 for Bacteroides thetaiotaomicron VPI-5482

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Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Trimethoprim (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 11 genes in this experiment

For stress Trimethoprim in Bacteroides thetaiotaomicron VPI-5482

For stress Trimethoprim across organisms

SEED Subsystems

Subsystem #Specific
Butanol Biosynthesis 1
De Novo Purine Biosynthesis 1
Fermentations: Mixed acid 1
Glutamate dehydrogenases 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Methicillin resistance in Staphylococci 1
Multidrug Resistance Efflux Pumps 1
Purine Utilization 1
Teichoic and lipoteichoic acids biosynthesis 1
Transport of Zinc 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate biosynthesis III 1 1 1
L-glutamate degradation I 1 1 1
reductive monocarboxylic acid cycle 2 2 1
pyruvate fermentation to acetate IV 3 3 1
pyruvate fermentation to ethanol I 3 2 1
L-alanine degradation II (to D-lactate) 3 2 1
ethene biosynthesis IV (engineered) 3 1 1
5-aminoimidazole ribonucleotide biosynthesis I 5 5 1
5-aminoimidazole ribonucleotide biosynthesis II 5 5 1
enterobacterial common antigen biosynthesis 5 1 1
Salmonella enterica serotype O:54 O antigen biosynthesis 5 1 1
Escherichia coli serotype O:15 O antigen biosynthesis 5 1 1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 6 1
L-threonine degradation I 6 5 1
Escherichia coli serotype O:50 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:56 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:149/Shigella boydii serotype O1 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:77/Salmonella enterica serotype O:6,14 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:13 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:177 O antigen biosynthesis 6 1 1
L-glutamate and L-glutamine biosynthesis 7 5 1
Escherichia coli serotype O:71/Salmonella enterica serotype O:28ac O antigen biosynthesis 7 3 1
Salmonella enterica serotype O:39 O antigen biosynthesis 7 2 1
L-glutamate degradation XI (reductive Stickland reaction) 7 2 1
Escherichia coli serotype O:157/Salmonella enterica serotype O:30 O antigen biosynthesis 7 2 1
Salmonella enterica serotype O:18 O antigen biosynthesis 7 2 1
Escherichia coli serotype O:152 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:2 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:7 O antigen biosynthesis 7 1 1
Salmonella enterica serotype O:6,7 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:111/Salmonella enterica serotype O:35 O antigen biosynthesis 7 1 1
4-aminobutanoate degradation V 7 1 1
Escherichia coli serotype O:1B/Salmonella enterica serotype O:42 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:85/Salmonella enterica serotype O:17 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:104 O antigen biosynthesis 8 3 1
Escherichia coli serotype O:107 O antigen biosynthesis 8 3 1
Escherichia coli serotype O:117 O antigen biosynthesis 8 3 1
Shigella boydii serotype 6 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:21/Salmonella enterica serotype O:38 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:86 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:128 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:51/Salmonella enterica serotype O:57 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:55/Salmonella enterica serotype O:50 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:127 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:49 O antigen biosynthesis 8 1 1
Escherichia coli serotype O:52 O antigen biosynthesis 8 1 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 7 1
Escherichia coli serotype O:169 O antigen biosynthesis 9 2 1
Escherichia coli serotype O:183/Shigella boydii serotype O:10 O antigen biosynthesis 9 2 1
Escherichia coli serotype O:8 O antigen biosynthesis 9 1 1
superpathway of enterobacterial common antigen biosynthesis 10 4 1
L-glutamate degradation V (via hydroxyglutarate) 10 2 1
poly(3-O-β-D-glucopyranosyl-N-acetylgalactosamine 1-phosphate) wall teichoic acid biosynthesis 10 1 1
Escherichia coli serotype O:9 O antigen biosynthesis 10 1 1
Escherichia coli serotype O:9a O antigen biosynthesis 10 1 1
poly(glycerol phosphate) wall teichoic acid biosynthesis 11 2 1
poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis) 12 1 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 10 1
poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus) 14 1 1
mixed acid fermentation 16 12 1
superpathway of L-threonine metabolism 18 13 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
methylaspartate cycle 19 11 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 1
H. pylori 26695 O-antigen biosynthesis 21 1 1
superpathway of N-acetylneuraminate degradation 22 16 1
superpathway of purine nucleotides de novo biosynthesis II 26 23 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 1