Experiment set10IT015 for Bacteroides thetaiotaomicron VPI-5482

Varel_Bryant_medium_Glucose with Chlorpromazine hydrochloride 0.1 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Chlorpromazine hydrochloride (0.1 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 6 genes in this experiment

For stress Chlorpromazine hydrochloride in Bacteroides thetaiotaomicron VPI-5482

For stress Chlorpromazine hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
ATP-dependent RNA helicases, bacterial	1
D-ribose utilization	1
Deoxyribose and Deoxynucleoside Catabolism	1
YeiH	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
ribose phosphorylation	2	1	1
2-deoxy-D-ribose degradation I	3	2	1