Experiment set10IT011 for Pseudomonas fluorescens FW300-N1B4

Compare to:

Nitrate reduction, replicate C

200 most detrimental genes:

  gene name fitness t score description  
Pf1N1B4_5619 +4.9 35.9 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf1N1B4_3501 +4.3 35.0 BarA sensory histidine kinase (= VarS = GacS) compare
Pf1N1B4_3115 +3.9 29.5 RNA polymerase sigma factor RpoS compare
Pf1N1B4_1185 +3.8 19.1 Lactate-responsive regulator LldR in Enterobacteria, GntR family compare
Pf1N1B4_5924 +2.2 12.6 hypothetical protein compare
Pf1N1B4_218 +2.2 16.6 Periplasmic binding protein compare
Pf1N1B4_5224 +2.1 6.0 tRNA-Arg-CCT compare
Pf1N1B4_5926 +2.1 15.1 hypothetical protein compare
Pf1N1B4_5925 +2.0 13.0 hypothetical protein compare
Pf1N1B4_1619 +1.9 14.8 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf1N1B4_2917 +1.8 11.8 FIG140336: TPR domain protein compare
Pf1N1B4_2381 +1.7 12.3 Protein yceI precursor compare
Pf1N1B4_2169 +1.6 5.6 FIG00953808: hypothetical protein compare
Pf1N1B4_2380 +1.6 13.3 Cytochrome B561 compare
Pf1N1B4_287 +1.6 8.5 Possible periplasmic thiredoxin compare
Pf1N1B4_3325 +1.6 3.9 Sigma factor RpoE negative regulatory protein RseB precursor compare
Pf1N1B4_407 +1.5 3.7 ADP-ribosylglycohydrolase YegU (EC 3.2.-.-) compare
Pf1N1B4_5376 +1.5 1.5 hypothetical protein compare
Pf1N1B4_1190 +1.5 10.0 Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing compare
Pf1N1B4_3549 +1.4 5.2 YciL protein compare
Pf1N1B4_4357 +1.4 5.3 Putrescine utilization regulator compare
Pf1N1B4_288 +1.4 9.2 FIG00958794: hypothetical protein compare
Pf1N1B4_5508 +1.4 2.1 hypothetical protein compare
Pf1N1B4_5055 +1.3 6.6 (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-) compare
Pf1N1B4_108 +1.3 2.4 FIG00954456: hypothetical protein compare
Pf1N1B4_1474 +1.3 7.4 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) compare
Pf1N1B4_866 +1.3 1.7 Iron-sulfur cluster regulator IscR compare
Pf1N1B4_3911 +1.3 5.1 Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family compare
Pf1N1B4_286 +1.2 7.4 2-dehydropantoate 2-reductase (EC 1.1.1.169) compare
Pf1N1B4_629 +1.2 2.3 Queuosine Biosynthesis QueE Radical SAM compare
Pf1N1B4_2837 +1.2 5.9 FIG00956650: hypothetical protein compare
Pf1N1B4_5737 +1.2 2.3 Nitrate/nitrite transporter compare
Pf1N1B4_1668 +1.2 4.0 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf1N1B4_3316 +1.2 4.0 Tricarboxylate transport transcriptional regulator TctD compare
Pf1N1B4_1115 +1.2 6.4 Dna binding response regulator PrrA (RegA) compare
Pf1N1B4_224 +1.2 1.9 Isohexenylglutaconyl-CoA hydratase compare
Pf1N1B4_2114 +1.1 5.1 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf1N1B4_2401 +1.1 1.3 Lipoprotein, putative compare
Pf1N1B4_157 +1.1 2.9 ThiJ/PfpI family protein compare
Pf1N1B4_4448 +1.1 2.0 COGs COG2343 compare
Pf1N1B4_4855 +1.1 4.0 Response regulator compare
Pf1N1B4_3451 +1.1 3.2 Aspartokinase (EC 2.7.2.4) compare
Pf1N1B4_3619 +1.1 3.0 Flagellar biosynthesis protein FliQ compare
Pf1N1B4_441 +1.1 2.9 hypothetical protein compare
Pf1N1B4_2163 +1.1 2.7 hypothetical protein compare
Pf1N1B4_4303 +1.1 3.3 DipZ protein compare
Pf1N1B4_4897 +1.0 2.2 Transcriptional regulator, LysR family compare
Pf1N1B4_4657 +1.0 3.0 Oxidoreductase probably involved in sulfite reduction compare
Pf1N1B4_2359 +1.0 2.5 Chemotaxis signal transduction protein compare
Pf1N1B4_3069 +1.0 2.9 FIG00954614: hypothetical protein compare
Pf1N1B4_5916 +1.0 2.6 Similar to non-heme chloroperoxidase, sll5080 homolog compare
Pf1N1B4_1866 +1.0 1.8 Methionine ABC transporter ATP-binding protein compare
Pf1N1B4_5973 +1.0 3.3 Probable permease of ABC transporter compare
Pf1N1B4_611 +1.0 3.2 CoA transferase, CAIB/BAIF family compare
Pf1N1B4_2190 +1.0 5.9 Regulator of sigma D compare
Pf1N1B4_5289 +1.0 2.5 hypothetical protein compare
Pf1N1B4_3191 +1.0 1.6 FIG00953505: hypothetical protein compare
Pf1N1B4_1180 +1.0 0.8 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf1N1B4_2360 +1.0 1.4 hypothetical protein compare
Pf1N1B4_516 +1.0 2.2 Sensor histidine kinase compare
Pf1N1B4_5378 +1.0 2.3 Phage protein compare
Pf1N1B4_4729 +1.0 1.9 Hemolysin-type calcium-binding region compare
Pf1N1B4_1884 +1.0 6.1 Sensory box histidine kinase compare
Pf1N1B4_205 +1.0 3.0 Pyoverdin biosynthesis protein PvdH, L-2,4-diaminobutyrate:2-oxoglutarate aminotransferase (EC 2.6.1.76) compare
Pf1N1B4_4830 +1.0 2.9 Membrane fusion component of tripartite multidrug resistance system compare
Pf1N1B4_1188 +1.0 6.7 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf1N1B4_3836 +1.0 1.4 Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4) compare
Pf1N1B4_3599 +1.0 2.9 Flagellar regulatory protein FleQ compare
Pf1N1B4_3627 +1.0 1.0 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf1N1B4_381 +1.0 1.5 hypothetical protein compare
Pf1N1B4_4560 +1.0 1.9 Transcriptional regulator, MerR family compare
Pf1N1B4_5797 +1.0 1.2 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase (EC 1.3.1.25) compare
Pf1N1B4_290 +1.0 8.8 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_4234 +1.0 2.4 putative transport protein compare
Pf1N1B4_3420 +1.0 2.0 Transcriptional regulator, AraC family compare
Pf1N1B4_3494 +1.0 2.3 Flavoprotein WrbA compare
Pf1N1B4_324 +0.9 2.9 Glycosyl transferase, group 2 family protein compare
Pf1N1B4_507 +0.9 2.1 FIG00956448: hypothetical protein compare
Pf1N1B4_1891 +0.9 2.3 ABC-type transport system involved in resistance to organic solvents, periplasmic component USSDB6C compare
Pf1N1B4_2776 +0.9 1.4 Transcriptional regulator, GntR family compare
Pf1N1B4_1116 +0.9 5.8 Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-) compare
Pf1N1B4_2227 +0.9 1.3 FIG019278: hypothetical protein compare
Pf1N1B4_4669 +0.9 1.1 Heavy metal RND efflux outer membrane protein, CzcC family compare
Pf1N1B4_3141 +0.9 1.7 FIG00959768: hypothetical protein compare
Pf1N1B4_3129 +0.9 1.0 Prophage baseplate assembly protein V compare
Pf1N1B4_2637 +0.9 4.2 dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) compare
Pf1N1B4_2458 +0.9 1.6 Sarcosine oxidase gamma subunit (EC 1.5.3.1) compare
Pf1N1B4_3979 +0.9 3.2 MFS permease protein compare
Pf1N1B4_3794 +0.9 3.1 hypothetical protein compare
Pf1N1B4_740 +0.9 1.8 Leucyl-tRNA synthetase compare
Pf1N1B4_1189 +0.9 3.5 Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG compare
Pf1N1B4_4414 +0.9 2.4 Bll6261 protein compare
Pf1N1B4_904 +0.9 1.3 DNA-binding protein HU-beta compare
Pf1N1B4_3785 +0.9 1.6 FOG: Ankyrin repeat compare
Pf1N1B4_5854 +0.9 4.0 FIG00960144: hypothetical protein compare
Pf1N1B4_2069 +0.9 3.2 Outer membrane protein ImpK/VasF, OmpA/MotB domain compare
Pf1N1B4_2820 +0.9 1.2 FIG00953647: hypothetical protein compare
Pf1N1B4_2853 +0.9 1.4 Pantoate--beta-alanine ligase (EC 6.3.2.1) compare
Pf1N1B4_1334 +0.9 1.8 FIG00955840: hypothetical protein compare
Pf1N1B4_102 +0.9 1.3 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf1N1B4_3729 +0.9 1.2 Quaternary ammonium compound-resistance protein SugE compare
Pf1N1B4_5095 +0.9 1.3 hypothetical protein compare
Pf1N1B4_1527 +0.9 1.9 Queuosine Biosynthesis QueC ATPase compare
Pf1N1B4_4192 +0.9 1.6 Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase compare
Pf1N1B4_3811 +0.9 1.6 Hydantoin racemase (EC 5.1.99.-) compare
Pf1N1B4_5041 +0.9 2.2 Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4) compare
Pf1N1B4_3149 +0.9 1.2 hypothetical protein compare
Pf1N1B4_248 +0.9 2.8 EAL-domain protein compare
Pf1N1B4_2783 +0.9 1.5 Transcription elongation factor GreB-related protein compare
Pf1N1B4_230 +0.8 0.6 FIG00953472: hypothetical protein compare
Pf1N1B4_5591 +0.8 1.9 Vanillin dehydrogenase (Hydroxybenzaldehyde dehydrogenase) (EC 1.2.1.28) compare
Pf1N1B4_2733 +0.8 1.8 DNA polymerase III alpha subunit (EC 2.7.7.7) compare
Pf1N1B4_3508 +0.8 1.4 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf1N1B4_5353 +0.8 2.5 hypothetical protein compare
Pf1N1B4_2168 +0.8 3.5 FIG004064: hypothetical protein compare
Pf1N1B4_359 +0.8 2.6 GGDEF domain protein compare
Pf1N1B4_339 +0.8 1.0 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf1N1B4_1055 +0.8 2.3 Phosphate acetyltransferase (EC 2.3.1.8) compare
Pf1N1B4_5279 +0.8 2.6 hypothetical protein compare
Pf1N1B4_3607 +0.8 2.7 Flagellum-specific ATP synthase FliI compare
Pf1N1B4_5284 +0.8 2.3 hypothetical protein compare
Pf1N1B4_4568 +0.8 2.0 FIG00793915: hypothetical protein compare
Pf1N1B4_5927 +0.8 7.0 Capsular polysaccharide biosynthesis protein compare
Pf1N1B4_5718 +0.8 1.7 Transcriptional regulator, Cro/CI family compare
Pf1N1B4_476 +0.8 2.0 ABC-type multidrug transport system, ATPase component compare
Pf1N1B4_2400 +0.8 1.8 FIG00955006: hypothetical protein compare
Pf1N1B4_2437 +0.8 3.6 Microsomal dipeptidase (EC 3.4.13.19) compare
Pf1N1B4_1336 +0.8 0.7 Putative multicopper oxidases compare
Pf1N1B4_4531 +0.8 1.6 FIG01221261: hypothetical protein compare
Pf1N1B4_5860 +0.8 2.1 Two-component response regulator compare
Pf1N1B4_3552 +0.8 2.3 Hypothetical YciO protein, TsaC/YrdC paralog compare
Pf1N1B4_5549 +0.8 2.1 hypothetical protein compare
Pf1N1B4_1882 +0.8 7.3 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) compare
Pf1N1B4_3111 +0.8 1.6 tRNA pseudouridine 13 synthase (EC 4.2.1.-) compare
Pf1N1B4_2061 +0.8 1.8 hypothetical protein compare
Pf1N1B4_4390 +0.8 1.8 Transcriptional regulator, LacI family compare
Pf1N1B4_485 +0.8 1.7 L-lysine permease compare
Pf1N1B4_5105 +0.8 2.2 Peptide ABC transporter, ATP-binding protein compare
Pf1N1B4_4824 +0.8 1.9 Gamma-glutamyl-putrescine oxidase (EC1.4.3.-) compare
Pf1N1B4_3153 +0.8 1.6 hypothetical protein compare
Pf1N1B4_708 +0.8 2.9 Chromosome initiation inhibitor compare
Pf1N1B4_3364 +0.8 2.0 Cyn operon transcriptional activator compare
Pf1N1B4_3117 +0.8 4.2 DNA mismatch repair protein MutS compare
Pf1N1B4_296 +0.8 1.9 ABC transporter ATP-binding protein YvcR compare
Pf1N1B4_3621 +0.8 2.8 Flagellar biosynthesis protein FlhB compare
Pf1N1B4_5904 +0.8 1.6 FIG00955320: hypothetical protein compare
Pf1N1B4_1441 +0.8 4.7 Putative esterase, FIGfam005057 compare
Pf1N1B4_1984 +0.8 2.2 Threonyl-tRNA synthetase (EC 6.1.1.3) compare
Pf1N1B4_3614 +0.8 2.3 Flagellar biosynthesis protein FliL compare
Pf1N1B4_1475 +0.8 3.6 FIG00962376: hypothetical protein compare
Pf1N1B4_5043 +0.8 1.3 hypothetical protein compare
Pf1N1B4_2085 +0.8 1.6 Uncharacterized protein similar to VCA0109 compare
Pf1N1B4_2334 +0.8 2.2 Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB compare
Pf1N1B4_2382 +0.8 5.4 ATP-dependent RNA helicase RhlE compare
Pf1N1B4_5898 +0.8 1.3 hypothetical protein compare
Pf1N1B4_3176 +0.8 1.8 Succinylglutamate desuccinylase/aspartoacylase compare
Pf1N1B4_1675 +0.7 1.6 Transcriptional regulator compare
Pf1N1B4_842 +0.7 2.8 putative membrane protein compare
Pf1N1B4_5061 +0.7 1.4 Valyl-tRNA synthetase compare
Pf1N1B4_1679 +0.7 1.2 Membrane-fusion protein compare
Pf1N1B4_2925 +0.7 1.1 hypothetical protein compare
Pf1N1B4_4759 +0.7 1.1 Proteinase inhibitor I11, ecotin precursor compare
Pf1N1B4_5972 +0.7 1.1 Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1) compare
Pf1N1B4_2193 +0.7 1.8 hypothetical protein compare
Pf1N1B4_890 +0.7 2.1 DNA recombination and repair protein RecO compare
Pf1N1B4_733 +0.7 1.0 Methionyl-tRNA synthetase (EC 6.1.1.10) compare
Pf1N1B4_2436 +0.7 2.8 FIG00953563: hypothetical protein compare
Pf1N1B4_4851 +0.7 1.7 Transcriptional regulator of various polyols utilization, AraC family compare
Pf1N1B4_1248 +0.7 2.5 Probable short-chain dehydrogenase compare
Pf1N1B4_4250 +0.7 2.2 Methyltransferase (EC 2.1.1.-) compare
Pf1N1B4_4218 +0.7 1.4 Multidrug resistance protein A compare
Pf1N1B4_4320 +0.7 1.8 Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1) compare
Pf1N1B4_3622 +0.7 3.2 Flagellar biosynthesis protein FlhA compare
Pf1N1B4_4293 +0.7 1.6 hypothetical protein compare
Pf1N1B4_131 +0.7 1.2 hypothetical protein compare
Pf1N1B4_4241 +0.7 3.4 Dienelactone hydrolase family compare
Pf1N1B4_3413 +0.7 1.5 Transcriptional regulator, LysR family compare
Pf1N1B4_619 +0.7 0.9 Sigma-54 dependent transcriptional regulator/sensory box protein compare
Pf1N1B4_1669 +0.7 3.7 ADP compounds hydrolase NudE (EC 3.6.1.-) compare
Pf1N1B4_5261 +0.7 1.3 hypothetical protein compare
Pf1N1B4_3841 +0.7 3.5 Nitrate ABC transporter, nitrate-binding protein compare
Pf1N1B4_1555 +0.7 4.5 Polyhydroxyalkanoic acid synthase compare
Pf1N1B4_1722 +0.7 2.3 transcriptional regulator, Crp/Fnr family compare
Pf1N1B4_1350 +0.7 1.1 GCN5-related N-acetyltransferase compare
Pf1N1B4_1051 +0.7 3.2 Glutathione peroxidase family protein compare
Pf1N1B4_1705 +0.7 1.2 SAM-dependent methyltransferases compare
Pf1N1B4_5616 +0.7 2.5 Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16) compare
Pf1N1B4_5109 +0.7 2.5 Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases compare
Pf1N1B4_1403 +0.7 3.2 3'-to-5' exoribonuclease RNase R compare
Pf1N1B4_2476 +0.7 3.2 Predicted signal-transduction protein containing cAMP-binding and CBS domains compare
Pf1N1B4_4826 +0.7 1.2 glutamine synthetase family protein compare
Pf1N1B4_4143 +0.7 1.7 hypothetical protein compare
Pf1N1B4_2680 +0.7 4.9 MORN repeat family protein compare
Pf1N1B4_242 +0.7 2.7 Glutathione-regulated potassium-efflux system protein KefB compare
Pf1N1B4_815 +0.7 3.1 iron-chelator utilization protein compare
Pf1N1B4_3975 +0.7 1.6 Transcriptional regulator, IclR family compare
Pf1N1B4_328 +0.7 2.5 Glucosyltransferase (side chain biosynthesis) (EC 2.4.1.-) compare
Pf1N1B4_389 +0.7 3.9 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_5460 +0.7 1.1 TolA protein compare
Pf1N1B4_1444 +0.7 3.1 ADP-ribose pyrophosphatase (EC 3.6.1.13) compare


Specific Phenotypes

For 15 genes in this experiment

For denitrifying Sodium pyruvate in Pseudomonas fluorescens FW300-N1B4

For denitrifying Sodium pyruvate across organisms