Experiment set10IT009 for Pseudomonas fluorescens FW300-N1B4
Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
Group: denitrifyingMedia: Chakraborty_Basal + Sodium pyruvate (15 mM) + Sodium nitrate (30 mM)
Culturing: pseudo1_N1B4_ML1, 150mL Serum Bottle, Anaerobic, at 27 (C), shaken=0 rpm
Growth: about 2.1 generations
By: Alex Aaring on 13-Jan-17
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 0.3792 g/L Sodium phosphate monobasic, 0.9713 g/L Disodium phosphate, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 7 genes in this experiment
For denitrifying Sodium pyruvate in Pseudomonas fluorescens FW300-N1B4
For denitrifying Sodium pyruvate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Fermentations: Mixed acid | 1 |
| Glycerolipid and Glycerophospholipid Metabolism in Bacteria | 1 |
| Lactate utilization | 1 |
| TCA Cycle | 1 |
| Terminal cytochrome C oxidases | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Citrate cycle (TCA cycle)
- Fatty acid metabolism
- Oxidative phosphorylation
- Glycine, serine and threonine metabolism
- Tyrosine metabolism
- Glutathione metabolism
- 1- and 2-Methylnaphthalene degradation
- 3-Chloroacrylic acid degradation
- Reductive carboxylate cycle (CO2 fixation)
- Retinol metabolism
- Metabolism of xenobiotics by cytochrome P450
- Drug metabolism - cytochrome P450
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: