Experiment set10IT008 for Bacteroides thetaiotaomicron VPI-5482

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Varel_Bryant_medium_Glucose with Anhydrotetracycline hydrochloride 0.00078125 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Anhydrotetracycline hydrochloride (0.00078125 mM) + Dimethyl Sulfoxide (0.0078125 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

None in this experiment

For Bacteroides thetaiotaomicron VPI-5482 in stress experiments

For stress Anhydrotetracycline hydrochloride across organisms