Experiment set10IT007 for Bacteroides thetaiotaomicron VPI-5482

Varel_Bryant_medium_Glucose with Thioridazine hydrochloride 0.025 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Thioridazine hydrochloride (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 3 genes in this experiment

For stress Thioridazine hydrochloride in Bacteroides thetaiotaomicron VPI-5482

For stress Thioridazine hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
D-ribose utilization	1
Deoxyribose and Deoxynucleoside Catabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
ribose phosphorylation	2	1	1
2-deoxy-D-ribose degradation I	3	2	1