Experiment set10H9 for Pseudomonas stutzeri RCH2

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D-Maltose monohydrate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + D-Maltose monohydrate (20 mM), pH=7.2
Culturing: psRCH2_ML7c, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.5 generations
By: Kelly on 2/24/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 13 genes in this experiment

For carbon source D-Maltose monohydrate in Pseudomonas stutzeri RCH2

For carbon source D-Maltose monohydrate across organisms

SEED Subsystems

Subsystem #Specific
Maltose and Maltodextrin Utilization 7
Alanine biosynthesis 1
Bacterial Chemotaxis 1
Branched-Chain Amino Acid Biosynthesis 1
Glutathione: Non-redox reactions 1
Glycogen metabolism 1
Isoleucine degradation 1
Leucine Biosynthesis 1
Leucine Degradation and HMG-CoA Metabolism 1
Pyruvate Alanine Serine Interconversions 1
Trehalose Biosynthesis 1
Trehalose Uptake and Utilization 1
Type IV pilus 1
Valine degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine biosynthesis I 2 2 1
L-isoleucine biosynthesis V 3 3 1
L-leucine degradation III 3 2 1
L-valine degradation III (oxidative Stickland reaction) 3 2 1
L-leucine degradation V (oxidative Stickland reaction) 3 2 1
L-valine degradation II 3 2 1
L-isoleucine degradation III (oxidative Stickland reaction) 3 2 1
L-isoleucine degradation II 3 2 1
starch degradation I 3 1 1
L-valine biosynthesis 4 4 1
superpathway of L-alanine biosynthesis 4 4 1
glycogen biosynthesis I (from ADP-D-Glucose) 4 3 1
4-hydroxy-2-nonenal detoxification 4 1 1
glycogen biosynthesis II (from UDP-D-Glucose) 4 1 1
starch biosynthesis 10 5 2
pentachlorophenol degradation 10 3 2
L-leucine degradation IV (reductive Stickland reaction) 5 1 1
superpathway of branched chain amino acid biosynthesis 17 17 3
L-leucine biosynthesis 6 6 1
L-leucine degradation I 6 5 1
L-isoleucine biosynthesis IV 6 5 1
L-isoleucine degradation I 6 5 1
L-isoleucine biosynthesis I (from threonine) 7 7 1
L-isoleucine biosynthesis III 7 4 1
glycogen degradation I 8 7 1
L-valine degradation I 8 6 1
L-isoleucine biosynthesis II 8 4 1
glycogen biosynthesis III (from α-maltose 1-phosphate) 8 4 1
glutathione-mediated detoxification I 8 3 1
gliotoxin biosynthesis 9 1 1
glutathione-mediated detoxification II 9 1 1
indole glucosinolate activation (intact plant cell) 12 3 1
camalexin biosynthesis 12 2 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of L-threonine metabolism 18 13 1
even iso-branched-chain fatty acid biosynthesis 34 24 1
odd iso-branched-chain fatty acid biosynthesis 34 24 1
anteiso-branched-chain fatty acid biosynthesis 34 24 1