Protein Info for Xcc-8004.478.1 in Xanthomonas campestris pv. campestris strain 8004
Annotation: Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 51% identical to PCXB_PSEPU: Protocatechuate 3,4-dioxygenase beta chain (pcaH) from Pseudomonas putida
KEGG orthology group: K00449, protocatechuate 3,4-dioxygenase, beta subunit [EC: 1.13.11.3] (inferred from 100% identity to xcb:XC_0379)MetaCyc: 51% identical to protocatechuate 3,4-dioxygenase beta subunit (Pseudomonas putida)
Protocatechuate 3,4-dioxygenase. [EC: 1.13.11.3]; 1.13.11.- [EC: 1.13.11.3]
Predicted SEED Role
"Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 1.13.11.3)
MetaCyc Pathways
- protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
- gallate degradation II (4/5 steps found)
- aromatic compounds degradation via β-ketoadipate (6/9 steps found)
- toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
- 4-sulfocatechol degradation (1/4 steps found)
- superpathway of aerobic toluene degradation (10/30 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (13/35 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.13.11.3
Use Curated BLAST to search for 1.13.11.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0H2X3G9 at UniProt or InterPro
Protein Sequence (243 amino acids)
>Xcc-8004.478.1 Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3) (Xanthomonas campestris pv. campestris strain 8004) MHDLPPDTPPGDVLLGYRRPRAGTQPAYLHAPYASTALRAPTRAPIAMPVTLSEVTGPRP SQITLGAHAADLTAGFAGEPLGERIIVSGRVLDENGAPVRNSVVEVWQCNAAGRYQHEGD RHDAPLDPNFRGTGQVLTDAHGRYAFKTIKPGAYPWRNHYNAWRPAHIHFSLHGDGIGQR LITQMYFPGDPLLAHDPIYNCVDDPLARDRMVSSFDWEHAVSEYALAYRFDIVLRGRKQT VWE