Protein Info for Xcc-8004.3980.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: 2-methylcitrate synthase (EC 2.3.3.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 transmembrane" amino acids 327 to 343 (17 residues), see Phobius details TIGR01800: 2-methylcitrate synthase/citrate synthase II" amino acids 16 to 383 (368 residues), 509.4 bits, see alignment E=2.4e-157 PF00285: Citrate_synt" amino acids 17 to 366 (350 residues), 413.2 bits, see alignment E=4.8e-128

Best Hits

Swiss-Prot: 81% identical to PRPC_CUPNE: 2-methylcitrate synthase (prpC) from Cupriavidus necator

KEGG orthology group: K01659, 2-methylcitrate synthase [EC: 2.3.3.5] (inferred from 100% identity to xca:xccb100_3313)

MetaCyc: 82% identical to 2-methylcitrate synthase (Cupriavidus necator)
2-methylcitrate synthase. [EC: 2.3.3.5]

Predicted SEED Role

"2-methylcitrate synthase (EC 2.3.3.5)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 2.3.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H2XBT7 at UniProt or InterPro

Protein Sequence (384 amino acids)

>Xcc-8004.3980.1 2-methylcitrate synthase (EC 2.3.3.5) (Xanthomonas campestris pv. campestris strain 8004)
MNDTVIPGFKPKKSVALSGTAAGNTALCTVGRSGNDLHYRGYDILDLATSSEFEEIAYLL
VHGKLPNSGELRGYKAKLRSLRGVPAAVKTALEQLPPSAHPMDVMRTGVSVLGCLQPEKD
DHNHPGARDIADKLMASLGSMLLYWYHYSHNGKRIEVETDDDSIGGHFLHLLHGFAPKES
WVRAMHTSLILYAEHEFNASTFASRVIAGTGSDMYSAIAGGIGALRGPKHGGANEVAFEV
QKRYDTPDEAEADIKARVERKEVVIGFGHPVYTVSDPRNKVIKDVARELSDEQGSRKMYD
IAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVLARTAGWSAHIIEQRVDG
KIIRPSANYTGPEDQAFVPLDQRA