Protein Info for Xcc-8004.2740.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Enoyl-CoA hydratase (EC 4.2.1.17) / 3,2-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02737: 3HCDH_N
  • amino acids 10 to 192 (183 residues), 138.1 bits, E=6.4e-44
• PF00725: 3HCDH
  • amino acids 195 to 293 (99 residues), 37 bits, E=8.3e-13
• PF00378: ECH_1
  • amino acids 487 to 619 (133 residues), 57 bits, E=3.9e-19
• PF16113: ECH_2
  • amino acids 494 to 617 (124 residues), 38.8 bits, E=1.7e-13

Best Hits

KEGG orthology group: K07516, 3-hydroxyacyl-CoA dehydrogenase [EC: 1.1.1.35] (inferred from 100% identity to xcb:XC_2205)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / 3,2-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.3.3.8)

MetaCyc Pathways

• oleate β-oxidation (31/35 steps found)
• adipate degradation (5/5 steps found)
• fatty acid β-oxidation I (generic) (6/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
• adipate biosynthesis (4/5 steps found)
• fatty acid β-oxidation III (unsaturated, odd number) (1/1 steps found)
• L-isoleucine degradation I (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• oleate β-oxidation (thioesterase-dependent, yeast) (1/2 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• oleate β-oxidation (reductase-dependent, yeast) (1/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• valproate β-oxidation (5/9 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (2/5 steps found)
• fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (2/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• oleate β-oxidation (isomerase-dependent, yeast) (1/4 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• phenylacetate degradation I (aerobic) (4/9 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (4/9 steps found)
• glycerol degradation to butanol (9/16 steps found)
• pyruvate fermentation to butanol I (3/8 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (5/11 steps found)
• superpathway of phenylethylamine degradation (5/11 steps found)
• 3-phenylpropanoate degradation (4/10 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• 2-methylpropene degradation (2/8 steps found)
• L-tryptophan degradation III (eukaryotic) (7/15 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (4/13 steps found)
• gallate degradation III (anaerobic) (2/11 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (3/13 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (3/14 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (5/17 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• platensimycin biosynthesis (6/26 steps found)
• Spodoptera littoralis pheromone biosynthesis (3/22 steps found)
• superpathway of testosterone and androsterone degradation (7/28 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (8/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (4/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.3.3.8

Sequence Analysis Tools

See A0A0H2X7E9 at UniProt or InterPro

Protein Sequence (790 amino acids)

>Xcc-8004.2740.1 Enoyl-CoA hydratase (EC 4.2.1.17) / 3,2-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) (Xanthomonas campestris pv. campestris strain 8004)
MSNPLLVRRAAVLGAGVMGAQIAAHLTNAGVDTVLFDLPAKEGPVDGIVLKAIANLGKLS
PAPLASKALAEAITPANYESGLEQLRDCDLIIEAIAERMDWKQDLYKKIAPFVSEHAVLA
SNTSGLGINKLSDVLPEQLRHRFCGVHFFNPPRYMHLAELIPAKGTDKAVLEGLESFLVT
TLGKGVVYAKDTPNFIGNRIGVFSILSTIHHTAEFGLGFDEVDGLTGPLVGRPKSATYRT
SDVVGLDTMAHVIKTMGDTLPDDPWHQYFKSPKWLEALIAKGALGQKVGAGIFRKVGKDI
VVLDLQKQDYRPADRSAAPEVIEILKIKNPAEKFAKLRESQHPQAQFLWATFRDLFHYSA
YHLADIAETARDVDLAIRWGYGWSLGPFETWQAAGWKQVAQWIAEDIASGKSMSSAPLPN
WVFDGRDGVHAAEGSYSPARDAKLPRSALPVYKRQRFPDPLLGEQFSPGETVFENDGVRM
WHDGDDIAVVSFKTKMNTVSDHVLNGLQEVVGRAEKDFAGLVIWQQKEPFSAGADLAGAL
GLLQAGKVDAFEALVANFQATSQRIKYAQVPVISAVRGLALGGGCEFQMHSAKTVASLES
YIGLVEAGVGLLPAGGGLKEIAARASVAAGPGGDVFAELKKTFETVAMAKVSNSAVNAQE
LGLLRTTDKIVFNSYEALHIAKAEARALAEGGYRAPLPARRIQVAGDVGIATFKMLLVNM
LEGRFISEYDYEIATRIATVVCGGEIDRGALVDEEWLLKLERKHFVELAQQEKTQARIGH
MLKTGKPLRN