Protein Info for Xcc-8004.2374.1 in Xanthomonas campestris pv. campestris strain 8004
Annotation: N-carbamoylputrescine amidase (3.5.1.53) / Aliphatic amidase AmiE (EC 3.5.1.4)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K12251, N-carbamoylputrescine amidase [EC: 3.5.1.53] (inferred from 100% identity to xcb:XC_1919)MetaCyc: 44% identical to At2g27450-monomer (Arabidopsis thaliana col)
N-carbamoylputrescine amidase. [EC: 3.5.1.53]
Predicted SEED Role
"N-carbamoylputrescine amidase (3.5.1.53) / Aliphatic amidase AmiE (EC 3.5.1.4)" in subsystem Arginine and Ornithine Degradation or Polyamine Metabolism (EC 3.5.1.4)
MetaCyc Pathways
- L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) (3/3 steps found)
- putrescine biosynthesis II (3/3 steps found)
- superpathway of polyamine biosynthesis II (6/8 steps found)
- acrylonitrile degradation I (1/2 steps found)
- indole-3-acetate biosynthesis III (bacteria) (1/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (1/2 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (1/3 steps found)
- superpathway of acrylonitrile degradation (1/3 steps found)
- indole-3-acetate biosynthesis II (3/12 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Cyanoamino acid metabolism
- Phenylalanine metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.4, 3.5.1.53
Use Curated BLAST to search for 3.5.1.4 or 3.5.1.53
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0H2X6Z4 at UniProt or InterPro
Protein Sequence (294 amino acids)
>Xcc-8004.2374.1 N-carbamoylputrescine amidase (3.5.1.53) / Aliphatic amidase AmiE (EC 3.5.1.4) (Xanthomonas campestris pv. campestris strain 8004) MTRHLLPVALIQERNHGDAEANLAVIESRVAEAAAQGAKLVLLQELHNGAYFCQHESVDV FDLAEPIPGPSTERLGALAKQHGVVIVASLFERRAAGLYHNTAVVLEADGRLLGKYRKMH IPDDPGFYEKFYFTPGDLGFTPVDTSVGRLGVLVCWDQWYPEAARLMALAGAELLLYPTA IGWDPDDAQAEQERQRDAWVLSHRGHAVANGVPVLSCNRVGHEPSPIGASGIQFWGNSHV LGPQGEFIAEAGQDPTILVCDVDLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID