Protein Info for Xcc-8004.2176.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 838 PF00359: PTS_EIIA_2" amino acids 10 to 144 (135 residues), 91.6 bits, see alignment E=1.2e-29 PF00381: PTS-HPr" amino acids 164 to 241 (78 residues), 75.6 bits, see alignment E=6.6e-25 TIGR01003: phosphocarrier, HPr family" amino acids 164 to 241 (78 residues), 60.9 bits, see alignment E=8.8e-21 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 275 to 830 (556 residues), 477.9 bits, see alignment E=3.9e-147 PF05524: PEP-utilisers_N" amino acids 277 to 395 (119 residues), 69.6 bits, see alignment E=7e-23 PF00391: PEP-utilizers" amino acids 424 to 496 (73 residues), 68 bits, see alignment E=1.1e-22 PF02896: PEP-utilizers_C" amino acids 522 to 805 (284 residues), 379.2 bits, see alignment E=3.1e-117

Best Hits

Swiss-Prot: 100% identical to PTFAX_XANCP: Multiphosphoryl transfer protein (fruB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 100% identity to xcb:XC_1744)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H2X6K2 at UniProt or InterPro

Protein Sequence (838 amino acids)

>Xcc-8004.2176.1 Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) (Xanthomonas campestris pv. campestris strain 8004)
LSSPSIAPVTPDLVRLRATARDKDDAIAQAAQLLVAAGCVAPGYDASMRRREGLANTFLG
HGLAIPHGVGEDRHLVRRDGIAVLQLPEGVEWNPGQTTRLVVGIAAQSDTHITLLRRLTR
LIQDPAQLEALFTTDDPAVIVAALTGDRAPDTSAAPATDLAERFEWTIAYPSGLHARPAT
RWAETARGFSARAQVRAGDQAADAKSLVGLLQLGLRAGDSITVSAKGSDAPALLKRLRAV
MDSLTAQEKADAERAAQRRAAPVIGWTPPQAQPAIVGIGASPGVAIGIVHRLRAAQTEVA
DQPIGLGDGGVLLHDALTRTRQQLAAIQDDTQRRLGASDAAIFKAQAELLNDTDLITRTC
QLMVEGHGVAWSWHQAVEQIASGLAALGNPVLAGRAADLRDVGRRVLAQLDPAAAGAGLT
DLPEQPCILLAGDLSPSDTANLDTDCVLGLATAQGGPTSHTAILSRTLGLPALVAAGGQL
LDIEDGVTAIIDGSSGRLYINPSEQDLDAARTHIAEQQAIREREAAQRALPAETTDGHHI
DIGANVNLPEQVAMALTQGAEGVGLMRTEFLFLERGSTPTEDEQYQTYLAMARALDGRPL
IVRALDIGGDKQVAHLELPHEENPFLGVRGARLLLRRPDLLEPQLRALYRAAKDGARLSI
MFPMITSVPELISLREICARIRAELDAPELPIGIMIEVPAAAAQADVLARHADFFSIGTN
DLTQYVLAIDRQNPELAAEADSLHPAVLRMIRSTIDGARKHDRWVGVCGGLAGDPFGASL
LAGLGVQELSMTPNDIPAVKARLRGRALSALQQLAEQALQCETAEQVRALEAQREGQA