Protein Info for TX73_019155 in Rhodopseudomonas palustris CGA009

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details PF25917: BSH_RND" amino acids 58 to 243 (186 residues), 33.5 bits, see alignment E=3.1e-12 PF25885: HH_EMRA" amino acids 91 to 212 (122 residues), 104.4 bits, see alignment E=5.2e-34

Best Hits

Swiss-Prot: 46% identical to EMRA_ECOLI: Multidrug export protein EmrA (emrA) from Escherichia coli (strain K12)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 100% identity to rpa:RPA3697)

MetaCyc: 46% identical to multidrug efflux pump membrane fusion protein EmrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (385 amino acids)

>TX73_019155 efflux RND transporter periplasmic adaptor subunit (Rhodopseudomonas palustris CGA009)
MTTTNPTTPADPRAVRRKRLAWFGILLATTAVAAGAWAYLSNPALRHTDDAYVGGQLVQI
TSEMSGAVTRILADNTDWVAAGDVLVELDSTDARIELASAKAELAQAVRNVRGLFASDAR
SDADVHLRLAEFNKAQGDLNVKQTLISSGAVSRESLRHASDALSAAQAALASAQQARAQA
LALVNNSTLASNPPVQVAAERVRAAALAIERTRITAPVSGMVAQRSIQLGRRVGPGEKMM
TVVPLDRLWVDANFKEIQLQGVCAGQPAKVTADIYGRSITYHGTVRGIEAGTGAAFALLP
AQNATGNWIKVVQRAPVRISLDPSELIQHPLRIGMSAEVEVDTRTCDSTAAPAKRQEQSA
IHDARAAAADAVVRQVIADNIGADR