Protein Info for TX73_018540 in Rhodopseudomonas palustris CGA009

Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 666 transmembrane" amino acids 486 to 504 (19 residues), see Phobius details PF13667: ThiC-associated" amino acids 19 to 90 (72 residues), 83.8 bits, see alignment E=5.9e-28 TIGR00190: phosphomethylpyrimidine synthase" amino acids 154 to 606 (453 residues), 694.1 bits, see alignment E=3e-213 PF01964: ThiC_Rad_SAM" amino acids 155 to 602 (448 residues), 615.2 bits, see alignment E=5.7e-189

Best Hits

Swiss-Prot: 100% identical to THIC_RHOPA: Phosphomethylpyrimidine synthase (thiC) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 100% identity to rpa:RPA3577)

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (666 amino acids)

>TX73_018540 phosphomethylpyrimidine synthase ThiC (Rhodopseudomonas palustris CGA009)
MNIRSNPETTRAAVTTGALPSSKKIYATPASAPDLRVPLREIILSEGAGEPNLPVYDTSG
PYTDPTVVIDVNKGLPRPRTEWVKQRGGVEQYQGRDIKPEDNGNVGAAHAAKAFTAHHQP
LRGLDSPPPRGEGSGVGGATPAAFARGADHPPMITQYEFARRGIITKEMIYVAERENLGR
KQQLERAEAALADGESFGAAVPAFITPEFVRDEIARGRAIIPANINHGELEPMIIGRNFL
TKINANIGNSAVTSSVEEEVDKMVWAIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGT
VPIYQALEKCEGDPVKLTWELYKDTLIEQAEQGVDYFTIHAGVRLQYIHLTASRVTGIVS
RGGSIMAKWCLAHHKESFLYTHFDEICDLMRKYDVSFSLGDGLRPGSIADANDRAQFAEL
ETLGELTKIAWAKGCQVMIEGPGHVPMHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGY
DHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPDRNDVKTGVITYKIAAHAADLAKGHPAA
QLRDDALSRARFEFRWQDQFNLGLDPDTAQAFHDETLPKDAHKVAHFCSMCGPKFCSMKI
TQDVRDYAAGLGDNEKAALYPVGHAGMTISGTIEDGMAQMSAKFKEMGSSVYLDAEKVKE
SNKALS