Protein Info for TX73_013390 in Rhodopseudomonas palustris CGA009

Annotation: lipoyl synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00510: lipoyl synthase
  • amino acids 26 to 310 (285 residues), 374.1 bits, E=2.7e-116
• PF04055: Radical_SAM
  • amino acids 81 to 244 (164 residues), 69.3 bits, E=2.4e-23

Best Hits

Swiss-Prot: 100% identical to LIPA_RHOPT: Lipoyl synthase (lipA) from Rhodopseudomonas palustris (strain TIE-1)

KEGG orthology group: K03644, lipoic acid synthetase [EC: 2.8.1.8] (inferred from 99% identity to rpx:Rpdx1_2884)

MetaCyc: 53% identical to lipoyl synthase (Escherichia coli K-12 substr. MG1655)
Lipoyl synthase. [EC: 2.8.1.8]

Predicted SEED Role

"Lipoate synthase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

• lipoate biosynthesis and incorporation I (2/2 steps found)
• lipoate biosynthesis and incorporation III (Bacillus) (2/3 steps found)
• lipoate biosynthesis and incorporation V (mammals) (2/3 steps found)
• lipoate biosynthesis and incorporation IV (yeast) (4/7 steps found)
• lipoate biosynthesis and incorporation II (1/3 steps found)

KEGG Metabolic Maps

• Lipoic acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.8

Protein Sequence (319 amino acids)

>TX73_013390 lipoyl synthase (Rhodopseudomonas palustris CGA009)
MVVLVDTVSANPVRPRHPEKAARPDALSPKKPDWIRVRAPTTRGYGETRGIVKENGLHTV
CEEAGCPNIGECWDKKHATFMIMGDTCTRACAFCNVKTGMPGALDANEPAYVAEATRKLG
LQHLVITSVDRDDLADGGAAHFAATIRAVREACPTTTIEILTPDFLRKDGALEVVVAAKP
DVFNHNLETVPSRYLSVRPGARYFHSIRLLQRVKELDPSIFTKSGIMVGLGEERHEVLQV
MDDLRSAEVDFLTIGQYLQPTRKHHAVMRYVTPDEFAGYQTTAYAKGFLMVSASPMTRSS
HHAGDDFAKLKAARAARAR