Protein Info for Synpcc7942_2324 in Synechococcus elongatus PCC 7942

Name: gshB
Annotation: glutathione synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 TIGR01380: glutathione synthase" amino acids 1 to 319 (319 residues), 469.9 bits, see alignment E=1.6e-145 PF02951: GSH-S_N" amino acids 2 to 128 (127 residues), 130 bits, see alignment E=6.9e-42 PF02955: GSH-S_ATP" amino acids 132 to 304 (173 residues), 249.7 bits, see alignment E=1.8e-78 PF08443: RimK" amino acids 143 to 315 (173 residues), 57.2 bits, see alignment E=2.7e-19

Best Hits

Swiss-Prot: 100% identical to GSHB_SYNE7: Glutathione synthetase (gshB) from Synechococcus elongatus (strain PCC 7942)

KEGG orthology group: K01920, glutathione synthase [EC: 6.3.2.3] (inferred from 100% identity to syc:syc1778_c)

MetaCyc: 64% identical to glutathione synthetase (Synechocystis sp. PCC 6803)
Glutathione synthase. [EC: 6.3.2.3]; 6.3.2.3 [EC: 6.3.2.3]; 6.3.2.3 [EC: 6.3.2.3]

Predicted SEED Role

"Glutathione synthetase (EC 6.3.2.3)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Heat shock dnaK gene cluster extended (EC 6.3.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See O32463 at UniProt or InterPro

Protein Sequence (323 amino acids)

>Synpcc7942_2324 glutathione synthetase (Synechococcus elongatus PCC 7942)
VKLAFLIDPIAALDPTHDSTVALMEAAQLRGHEIWIGEISDLSVHVGEPLGLLRPLKIEP
VQWLGDRWQVANPWFTAGEAKRRSLHDFAAVFMRKDPPVTTAYLYATYLLDLVDPKKTRV
VNSPEGLRHANEKMYALQFQSVVPRTLVSSNKAEIRAFLDELRAAVLKPLGGKAGEGILF
LDPGDRNFNSLVEISTQQGQLPVMVQQYLPEAKDGDKRIILVNGEPLGAVNRVPTGREFR
GNMAVGGRVEAVPITDRDREICAAVAPRLRQDGLFFVGIDVIGGYLTEVNVTSPTGIREI
DRLNGVSIGDQTIAALEALVNQG