Protein Info for Synpcc7942_1854 in Synechococcus elongatus PCC 7942

Name: cobJ
Annotation: precorrin-3 methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF11760: CbiG_N
  • amino acids 49 to 126 (78 residues), 87.9 bits, E=3.6e-29
• PF00590: TP_methylase
  • amino acids 320 to 531 (212 residues), 123.7 bits, E=9.9e-40
• TIGR01466: precorrin-3B C17-methyltransferase
  • amino acids 321 to 566 (246 residues), 308.5 bits, E=1.6e-96

Best Hits

KEGG orthology group: K13541, cobalamin biosynthesis protein CbiG / precorrin-3B C17-methyltransferase [EC: 2.1.1.131] (inferred from 100% identity to syc:syc2240_c)

Predicted SEED Role

"Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (31/36 steps found)
• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (14/15 steps found)
• cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (12/13 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.131

Sequence Analysis Tools

See Q31M35 at UniProt or InterPro

Protein Sequence (566 amino acids)

>Synpcc7942_1854 precorrin-3 methyltransferase (Synechococcus elongatus PCC 7942)
MTTIAIVILNELSFATATQVKQCLPDSMIYGLQKRTESADQTYQDFGETIRNLFQQGQTI
IGICSAGILIRALAPVLNNKWQEPAVLAIAEDGSSVVPLLGSLQGANQLAQQLAQVLQSH
AAITTTGAIRFQTTLLSPPSGYQLAFPSTAKTFIADLLNGQPVRIEGQAAWLKTTRLPFD
PEATYCIQVTHQLQQPQPQCLTYYSKNIGIFIQSQDNFDISETISAIDHLLSDNQIAPDA
IALIGVPFELANHATLQAIAAKFQVSVRLIRESPTATASDQARRILGSDIQILAENSTIA
IAIAEEPIQPEIIGQACGRVSIVGLGPGSAEWLTPEARQMLQHATDWVGYKTYLNLAEPW
RSPQTQRHDSDNRVELDRARYALDLAAIGRQVAVISSGDPGIYAMAAAVFEVLETAENLD
WQTLDIRICPGISAMQAAAAQVGAPLGHDFCTISLSDILKPWEIIAERITAAAQADFAIA
FYNPVSQTRRWQLEAARDLLLAWRSPETPVLLARNIGREKQSIQIIDLAELSADLVDMNT
VVLIGSSKTRQFESAGRHWLYTPRYY