Protein Info for Synpcc7942_1512 in Synechococcus elongatus PCC 7942

Name: crtQb
Annotation: zeta-carotene desaturase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR02732: 9,9'-di-cis-zeta-carotene desaturase" amino acids 2 to 474 (473 residues), 902.4 bits, see alignment E=3.6e-276 PF00890: FAD_binding_2" amino acids 3 to 37 (35 residues), 24.3 bits, see alignment 4.4e-09 PF13450: NAD_binding_8" amino acids 5 to 70 (66 residues), 49.2 bits, see alignment E=1.4e-16 PF01593: Amino_oxidase" amino acids 10 to 474 (465 residues), 250.4 bits, see alignment E=1.2e-77

Best Hits

Swiss-Prot: 100% identical to ZDS_SYNE7: Probable zeta-carotene desaturase (zds) from Synechococcus elongatus (strain PCC 7942)

KEGG orthology group: K00514, zeta-carotene desaturase [EC: 1.14.99.30] (inferred from 100% identity to syf:Synpcc7942_1512)

MetaCyc: 77% identical to 9,9'-dicis-zeta-carotene desaturase (Nostoc sp. PCC 7120 = FACHB-418)
RXN-12242 [EC: 1.3.5.6]; 1.3.5.6 [EC: 1.3.5.6]; 1.3.5.6 [EC: 1.3.5.6]

Predicted SEED Role

"Pro-zeta-carotene desaturase, prolycopene producing (EC 1.-.-.-)" in subsystem Carotenoids (EC 1.-.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.- or 1.14.99.30 or 1.3.5.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q31N27 at UniProt or InterPro

Protein Sequence (481 amino acids)

>Synpcc7942_1512 zeta-carotene desaturase (Synechococcus elongatus PCC 7942)
MRVAIVGAGLAGLAAAIDLVDAGHQVAIYDSRPFVGGKVGSWIDADGNHIEMGLHVFFFN
YANLFALMRKVGAFENLLPKAHTHTFINKGGEVGELDFRFPIGAPFNGLKAFFTTSQLTW
LDKLQNALALGTSPLVRGILDYEGAMKIIRALDRISFADWFRSHGGSEGSLKRMWNPIAY
ALGFIDTENISARCMLTVFQMFAAKTEASKLNLLAGSPAEYLHKPILDYIQARGATLHLR
RRVREIEYTETNGQTVVTGLQIADGDAVERVEADVYLAACDVPGIQRLLPEAWRKWSEFD
NIYKLDAVPVATVQLRFDGWVTELGDREKRHQLDHATGLDNLLYTADADFSCFTDLALSS
PKDYYRKGQGSLLQCVLTPGDPFIAMKNEDIAQHVLKQVHELFPSSRDLNMTWSNVVKLA
QSLYREAPGMDPFRPDQKTPIANFFLAGSYTQQDYIDSMEGATISGRRAAKAMLEAQAIA
A