Protein Info for Synpcc7942_0903 in Synechococcus elongatus PCC 7942

Name: acnB
Annotation: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00117: aconitate hydratase 2
  • amino acids 1 to 843 (843 residues), 1577.8 bits, E=0
• PF11791: Aconitase_B_N
  • amino acids 4 to 157 (154 residues), 215.2 bits, E=6.9e-68
• PF06434: Aconitase_2_N
  • amino acids 169 to 372 (204 residues), 336.6 bits, E=8e-105
• PF00330: Aconitase
  • amino acids 448 to 807 (360 residues), 135.2 bits, E=5.3e-43

Best Hits

Swiss-Prot: 77% identical to ACNB_SYNY3: Aconitate hydratase B (acnB) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K01682, aconitate hydratase 2 [EC: 4.2.1.3] (inferred from 79% identity to amr:AM1_1630)

MetaCyc: 67% identical to AcnB (Escherichia coli K-12 substr. MG1655)
2-methylisocitrate dehydratase. [EC: 4.2.1.99]; Aconitate hydratase. [EC: 4.2.1.99, 4.2.1.3]; 4.2.1.3 [EC: 4.2.1.99, 4.2.1.3]

Predicted SEED Role

"Aconitate hydratase 2 (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• ethene biosynthesis V (engineered) (24/25 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• TCA cycle I (prokaryotic) (7/10 steps found)
• TCA cycle III (animals) (7/10 steps found)
• glyoxylate cycle (4/6 steps found)
• TCA cycle II (plants and fungi) (6/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• TCA cycle VI (Helicobacter) (5/9 steps found)
• TCA cycle VII (acetate-producers) (5/9 steps found)
• 2-methylcitrate cycle I (2/5 steps found)
• superpathway of glyoxylate bypass and TCA (7/12 steps found)
• reductive TCA cycle I (6/11 steps found)
• 2-methylcitrate cycle II (2/6 steps found)
• reductive TCA cycle II (5/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (5/14 steps found)
• methylaspartate cycle (7/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.3 or 4.2.1.99

Sequence Analysis Tools

See Q31PT6 at UniProt or InterPro

Protein Sequence (861 amino acids)

>Synpcc7942_0903 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase (Synechococcus elongatus PCC 7942)
MLEAYRQAAAEREALGVPPLPLDADQTAALCELLQAPPVGEEATLLHLLRDRVPPGVDQA
AYVKATFLSAIAHGETTSPLIMPVEAVELLGTMIGGYNVAALIDLLKSADVAIATAAVAA
LSKTLLVYDAYNDVVALAETNAYAQQVLESWAKAEWFTSKPTLPEAITVTIFKVPGETNT
DDLSPATHATTRPDIPLHAQAMLETRLPGSLETIPVLKEKGYPLAYVGDVVGTGSSRKSA
INSVLWHIGEDIPFVPNKRSGGIILGGKIAPIFFNTAEDSGALPIECDVSALDTGMVVTI
YPYEGVIKDEAGTVLSTFSLKPDTILDEVRAGGRIPLLIGRSLTDKVRSQLGLPVSDVFV
RPQPPADTGKGFTLAQKMVGRACGLPGVRPGTSCEPIMTTVGSQDTTGPMTRDEMKELAC
LGFSADLVMQSFCHTAAYPKPVDIKTHKTLPDFIAQRGGVALKPGDGIIHSWLNRMLLPD
TVGTGGDSHTRFPLGISFPAGSGLVAFAAAIGAMPLDMPESVLVRFTGSLQPGITLRDVV
NAIPYQAIQQGLLTVSKENKVNVFSGRIMEIEGLPDLKLEQAFELTDATAERSCAGSTIK
LSEDTVAEYLRSNVALMKNMIARGYEDSRTLARRIRQMEDWLANPQLLSADEDAEYAAVI
EINLDELTEPILACPNDPDNVKKLSEVAGDPIHEIFIGSCMTNIGHYRAAAKVLEGEGQV
GGRLWICPPTRMDEDRLKEEGYYSTFAAAGARLEVPGCSLCMGNQARVADNTTVFSTSTR
NFNNRMGKGAQVYLGSAELAAVCALLGRIPTLEEYLKVAAEKINPFAADLYQYLNFDQLE
GFAEEGRVKSTEEVDRILTTV