Protein Info for Synpcc7942_0781 in Synechococcus elongatus PCC 7942

Name: ppsA
Annotation: phosphoenolpyruvate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 20 to 830 (811 residues), 1201.5 bits, E=0
• PF01326: PPDK_N
  • amino acids 32 to 382 (351 residues), 402.3 bits, E=2.5e-124
• PF00391: PEP-utilizers
  • amino acids 424 to 495 (72 residues), 93 bits, E=1e-30
• PF02896: PEP-utilizers_C
  • amino acids 521 to 829 (309 residues), 205.2 bits, E=2e-64

Best Hits

Swiss-Prot: 76% identical to PPSA_SYNY3: Phosphoenolpyruvate synthase (ppsA) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 77% identity to cyn:Cyan7425_2010)

MetaCyc: 58% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• gluconeogenesis I (12/13 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of N-acetylneuraminate degradation (15/22 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (12/19 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See Q31Q56 at UniProt or InterPro

Protein Sequence (838 amino acids)

>Synpcc7942_0781 phosphoenolpyruvate synthase (Synechococcus elongatus PCC 7942)
MTLTRFQPTAGNESRDQRFVLWFEEVGIDDIPLVGGKNASLGEMIRELLSKGVNVPLGFA
TTAAAFRFFLAGAGLEPQLRQLFADLDVEDVVNLRERGRQARNLILNTPFPAELETAIAT
AYQQLCDRYEPTPAVCDRLSGLDRDRCQRQFGSVDVAVRSSATAEDLPDASFAGQQETYL
NVRGVQAVIQACHRCFASLFTDRAISYRQIKGFDHFEVALSVGVQKMVRSDLAASGVMFS
IDTETGFRNAALVTAAYGLGENVVQGAVNPDEFFVFKPTLQQGFRPTLDKRIGSKEIRMV
YDEGGSKLTKNVSVAESDRQRFAISDDEVLQLAQWACIIEDHYSAKRGCFTPMDIEWAKD
GLSGELFIVQARPETVQSQRASNLLRSYQLQGSGSVLVEGRAVGEMIGQGTARVILDVHH
IADFQAGEVLVTNRTDPDWEPIMKKASAIVTNQGGRTCHAAIIAREMGIPAIVGCGTATT
AIATGADVTVSCAEGDQGRVYAGLLPFEVHETVLDDLPRPRTQILMNVGNPEEAFGLAAI
PCDGVGLARLEFIIANHIQAHPLALLHFDQLEDLFVQDQIAKLTANYDRKADFFVEKLAQ
GIGRIAAAFWPKPVIVRLSDFKSNEYANLLGGRQFEPREENPMIGWRGASRYYDPQYREA
FALECQAFRQVREGMGLTNVIPMVPFCRTPEEGQRVLAEMAANGLKRGENGLQVYVMCEL
PSNVICADAFSEVFDGFSIGSNDLTQLTLGLDRDSALVAALFDERNPAVKRMVAQAIQTV
KAAGRKIGICGQAPSDYPEFAEFLVEQGIDSISLNPDSVLKTLLAIAAVEARLETGRD