Protein Info for Synpcc7942_0711 in Synechococcus elongatus PCC 7942

Name: carB
Annotation: carbamoyl phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1077 (1076 residues), 1499.9 bits, E=0
• PF25596: CPSase_L_D1
  • amino acids 7 to 124 (118 residues), 167.7 bits, E=4.4e-53
  • amino acids 572 to 692 (121 residues), 157.3 bits, E=7.1e-50
• PF02786: CPSase_L_D2
  • amino acids 128 to 335 (208 residues), 241.5 bits, E=3.4e-75
  • amino acids 696 to 898 (203 residues), 129.6 bits, E=6.2e-41
• PF02787: CPSase_L_D3
  • amino acids 426 to 503 (78 residues), 86 bits, 7.3e-28
• PF02222: ATP-grasp
  • amino acids 703 to 869 (167 residues), 40.2 bits, E=1.5e-13
• PF07478: Dala_Dala_lig_C
  • amino acids 722 to 867 (146 residues), 32.7 bits, E=2.8e-11
• PF02142: MGS
  • amino acids 982 to 1065 (84 residues), 75.8 bits, 1.2e-24

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (15/18 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• superpathway of arginine and polyamine biosynthesis (12/17 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• L-arginine biosynthesis IV (archaea) (5/9 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See Q31QC6 at UniProt or InterPro

Protein Sequence (1097 amino acids)

>Synpcc7942_0711 carbamoyl phosphate synthase large subunit (Synechococcus elongatus PCC 7942)
MPRRQDIRKILLLGSGPIVIGQACEFDYSGTQACKALREEGYEVVLVNSNPATIMTDPET
ADRTYIEPLTPELVAQVIERERPDALLPTMGGQTALNLAVALAKNGTLDRFGVELIGAKL
EAIEKAEDRLLFKEAMERIGVKVCPSGIANNMAEAQAIAEQIGTYPLIIRPAFTMGGTGG
GIAYNQEEFELIVQSGLDASPVSQILVEQSLIGWKEFELEVMRDLADNVVIICSIENLDP
MGVHTGDSITVAPAQTLTDKEYQRLRDQAIAIIREIGVETGGSNIQFAINPQDGDVIVIE
MNPRVSRSSALASKATGFPIAKIAAKLAVGYSLDELKNDITRQTPASFEPTIDYVVTKIP
RFAFEKFPGTPAQLTTMMKSVGEAMAIGRTFPESFQKALRSLEIGRSGWGCDRPETLPTL
EQIKPQLRTPSPDRIFAVRQAMLLGLSDGDIYQLTGIDPWFLEKFREILEGEDFLKRASI
EQITPAQWLEVKQLGFSDRQIAFALGSSEEAVRQRRQQQGIKPVYKLVDTCAAEFEAYTP
YYYSCYESPRSHLAESGLTTIPAESEVRPSDRPKVMILGGGPNRIGQGIEFDYCCCHAAF
ALAADGYETIMVNSNPETVSTDYDTSDRLYFEPLTREDVLNILEAERPQGVIIQFGGQTP
LKLAVPLQTFLQTPEGQALGTQIWGTSPDSIDTAEDRERFEQILRQLDIAQPANGLARSP
EEALAIAHRIGYPTVVRPSYVLGGRAMEIVYSDAELERYMTEAVQVEPDHPILIDKFLEN
AIEVDVDALADRTGQVVIGGIMEHIEQAGIHSGDSACSLPTVSLNETVLETIRAWTTQLA
KALNVIGLMNIQFAVKDEQVYILEANPRASRTVPFVSKAIGRPLAKIAARLMSGQTLADL
GITSELVPSYLSVKEAVLPFDRFPGTDTLLGPEMRSTGEVMGIDVDFGRAFAKAELAASQ
RLPRQGTVFISVSDRDKAAIGPIAREFLQLGFRVVATGGTQKVLAEQRLSVEPILKVHEG
RPHVLDALKNDQIQLIINTPAGARAKVDDQIIRRAALDYKVPTVTTIAGAKATVEAIRAL
LEQPLQVKSLQDYQQQG