Protein Info for SMc04305 in Sinorhizobium meliloti 1021

Annotation: adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02283: CobU
  • amino acids 9 to 173 (165 residues), 209.3 bits, E=1.4e-66

Best Hits

Swiss-Prot: 81% identical to COBP_SINSX: Bifunctional adenosylcobalamin biosynthesis protein CobP (cobP) from Sinorhizobium sp.

KEGG orthology group: K02231, adenosylcobinamide kinase / adenosylcobinamide-phosphate guanylyltransferase [EC: 2.7.1.156 2.7.7.62] (inferred from 100% identity to smk:Sinme_1931)

MetaCyc: 81% identical to CobP (Pseudomonas denitrificans (nom. rej.))
Adenosylcobinamide-phosphate guanylyltransferase. [EC: 2.7.7.62]; Adenosylcobinamide kinase. [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]

Predicted SEED Role

"Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 2.7.7.62)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (30/33 steps found)
• adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
• adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
• superpathway of adenosylcobalamin salvage from cobinamide I (7/8 steps found)
• adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)
• superpathway of adenosylcobalamin salvage from cobinamide II (7/9 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (26/36 steps found)
• adenosylcobinamide-GDP salvage from assorted adenosylcobamides (1/2 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.156 or 2.7.7.62

Sequence Analysis Tools

See Q92P34 at UniProt or InterPro

Protein Sequence (175 amino acids)

>SMc04305 adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (Sinorhizobium meliloti 1021)
MDISNLGPTLVLGGARSGKSTFAEKLVEATGSPMHYLATGRAFDEEMQERIALHQATRQG
KGWTTHEEPLDLVGLLRRIDEPGRAVLIDCLTLWVTNLMMEERDMAAEFAALAAFLPEAR
SRLVFVSNEVGLGIVPENRMARDFRDHAGRLHQIVAEKSAEVYFVAAGLPLKMKG