Protein Info for SMc04146 in Sinorhizobium meliloti 1021

Annotation: phosphoketolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 817 PF09364: XFP_N" amino acids 22 to 381 (360 residues), 583.4 bits, see alignment E=2.3e-179 PF03894: XFP" amino acids 411 to 587 (177 residues), 288.1 bits, see alignment E=3.5e-90 PF09363: XFP_C" amino acids 598 to 799 (202 residues), 327.9 bits, see alignment E=3.1e-102

Best Hits

Swiss-Prot: 100% identical to PHK1_RHIME: Probable phosphoketolase 1 (R00106) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01636, [EC: 4.1.2.-] (inferred from 74% identity to gvi:glr0997)

Predicted SEED Role

"Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) @ Fructose-6-phosphate phosphoketolase (EC 4.1.2.22)" (EC 4.1.2.22, EC 4.1.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.2.-

Use Curated BLAST to search for 4.1.2.- or 4.1.2.22 or 4.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92T74 at UniProt or InterPro

Protein Sequence (817 amino acids)

>SMc04146 phosphoketolase (Sinorhizobium meliloti 1021)
MTSPRTARDVSSQEPLSAGPLSADELQRMDAYWRASNYLSVGQIYLLDNPLLSEPLKREH
IKPRLLGHWGTSPGLNMLYVHLNRVIKRDDLEMMYVIGPGHGGPSLVAHAYLEGTYSEVY
PDISQDAEGLRKLFKQFSFPGGIPSHVAPETPGSIHEGGELGYALSHAYGAAFDNPELIV
ACVVGDGEAETGPLATGWHGNKFLNPARDGCVLPILHLNGYKIANPCFLARIPREELQKF
FEGMGYAPLFVEGHDPADVHQQLAAALDTALADIRRIQTDARVNGNLKRPAWPMIVFRTP
KGWTCPAEIDGKKCEDYWRSHQVPMGDMDKPEHIRILEGWMKSYRPEELFDGDGRLTAEL
AALAPTGRRRMSDNPHANGGLLLRDLKMPDFRDYAVAVQSPGAATAESARVMGSYLRDVM
KLNLKSGNFRLFSPDENNSNRWQDVLEVTDRCFMADIYPEDDHLSPDGRLMEVLSEHQSQ
GWLEGYLLTGRHGFFSCYEAFIHIIDSMFNQHAKWLKVCNEIPWRRPIASLNYFLSSHVW
RQDHNGFSHQDPGFIDHVVNKKADIIRVYLPPDANTLLSVTDHCLRSRNYINVVVAGKQP
SPQWLTMDQAVKHCTEGLGIWEWASNDKGCEPDVVMACCGDVPTLETLAAVQLLREHLPE
LKVRVINVVNLMKLQPSGEHPHGLPDRDFDALFTKDKPIIFAFHGYPWLIHRLTYRRTNH
ANLHVRGYKEEGTTTTPFDMVVLNHLDRFHLVEDVIDRLPQLGARAAYFKQAIHERLIDH
RHHIEKYGEDMPVISGWKWGAGSAGKAQGTSTKGDNV