Protein Info for SMc04093 in Sinorhizobium meliloti 1021

Annotation: acetyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 20 to 643 (624 residues), 1034.4 bits, E=0
• PF16177: ACAS_N
  • amino acids 23 to 79 (57 residues), 74.2 bits, 9e-25
• PF00501: AMP-binding
  • amino acids 81 to 468 (388 residues), 295.5 bits, E=8.2e-92
• PF13193: AMP-binding_C
  • amino acids 531 to 609 (79 residues), 90.1 bits, E=2.2e-29

Best Hits

Swiss-Prot: 100% identical to ACSA_RHIME: Acetyl-coenzyme A synthetase (acsA1) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to smk:Sinme_3318)

MetaCyc: 66% identical to acetyl-CoA synthetase (AMP-forming) (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• β-alanine biosynthesis II (5/6 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• chitin deacetylation (3/4 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• acrylate degradation II (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• 2-methylcitrate cycle I (1/5 steps found)
• 2-methylcitrate cycle II (1/6 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1, 6.2.1.17

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Sequence Analysis Tools

See Q92KX2 at UniProt or InterPro

Protein Sequence (649 amino acids)

>SMc04093 acetyl-CoA synthetase (Sinorhizobium meliloti 1021)
MDVKTYPVLEAAKNRTLLDNATYLEWYRESVADPEKFWGEHGKRIEWFEPYTKVKNTSFE
GDVSIKWFEDGLTNVSYNCIDRHLKTHGEKTAIIWEGDNPYLDKKITYNELYDKVCRLAN
VLKEQGVKKGDRVTIYMPMIPEAAYAMLACARIGAIHSVVFGGFSPEALAGRIVDCESTF
VITCDEGVRGGKPVALKENTDTAIDIAARQHVTVSKVLVVRRTGGKVGWAPGRDLWYHQE
TAAAEPHCPPEKMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASMTHQYVFDYQDGDI
YWCTADVGWVTGHSYIVYGPLANAATTLMFEGVPNFPDAGRFWEVVDKHKVNIFYTAPTA
IRSLMGAGDDFVKRSSRSSLRLLGTVGEPINPEAWEWYYHVVGDERCPVVDTWWQTETGG
ILITPLPGATDLKPGSATRPFFGVQPQIVDSDGKVVDGAADGNLCITDSWPGQMRTVYGD
HERFIQTYFSTYKGKYFTGDGCRRDEDGYYWITGRVDDVLNVSGHRLGTAEVESALVSHN
LVSEAAVVGYPHPIKGQGIYCYVSLMAGEVGDDELRQALVKHVRSEIGPIATPDKIQFAP
GLPKTRSGKIMRRILRKIAEDDFGSLGDTSTLADPGVVDDLIANRQNRA