Protein Info for SMc04028 in Sinorhizobium meliloti 1021
Annotation: glutamate synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13
Use Curated BLAST to search for 1.4.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q92M45 at UniProt or InterPro
Protein Sequence (1574 amino acids)
>SMc04028 glutamate synthase (Sinorhizobium meliloti 1021) MTDHSPSRQIGLNLAQDAATAVKTPAFPSGLPRKQGLYDPRNEHDACGVGFVAHLKGEKS HQIVRDGLFMLENLTHRGAVGADPLMGDGAGILVQIPDRFFREEMAKQGVTLPKAGEYAV GYLFMPRDEALIAHFKEVISEVVAEEGQIVLGFRDVPVDNSSLSKAPDIAATEPHHVQVF IGAGREAASSDESERRLFTLRKVISNRIYAEADGGDLGFYIVSLSTTTIVYKGMFLAYQV GAYYKDLADERFQSAVALVHQRFSTNTFPSWKLAHPYRMVAHNGEINTLRGNVNWMAARQ ASVSSPLFGDDISKLWPISYEGQSDTACFDNALEFLVRGGYSLSHAVMMLIPEAWAGNQL MSPERKAFYEYHAALMEPWDGPAAVAFTDGRQIGATLDRNGLRPARYIVTSDDRVIMASE AGVLPVAEDKIVKKWRLQPGKMLLIDMEEGRIISDEEVKSSLAGKHPYRQWLDNTQLILE ELKPVEPRALRRDVSLIDRQQAFGYTHEDTKLLMSPMATTGQEAIGSMGTDTPISAMSDK PKLLYTYFKQNFAQVTNPPIDPIREELVMSLVSFIGPRPNILDHEGMAHAKRLEVRQPIL TNGDLEKIRSIGHTEDRFDTKTLDFTYDISRGAEGMLEMLDRLCERAEAAVKGGYNIIVL SDRQIGPDRVAIPALLATAAVHHHLIRKGLRTSVGLVVESGEPREIHHFCLLAGYGAEAI NPYLAFDTLVDMHKRGEFPKEVDEKEVVYRYIKAVGKGILKVMSKMGISTYQSYCGAQIF DAVGLSSALVGKYFFGTATTIEGIGLEEIAAETVARHKAAFGSDPVLANTLDIGGEYAFR MRGESHAWTPDAIASLQHAVRGNAEDRYREFSAMMNESASRMNTIRGLFTIKGAEEVGRK PIPIEEVEPAAEIVKRFSTGAMSFGSISREAHTTLAVAMNRIGGKSNTGEGGEESDRYLP LPDGSTNPERSAIKQIASGRFGVTTEYLVNADVLQIKVAQGAKPGEGGQLPGHKVDATVA KTRHSTPGVGLISPPPHHDIYSIEDLAQLIYDLKNVNPEADVSVKLVSEVGVGTVAAGVA KARADHITIAGFDGGTGASPLTSLKHAGSPWEIGLAETQQTLVLNGLRSRIALQVDGGLK TGRDVVIGALLGADEFGFATAPLIAAGCIMMRKCHLNTCPVGVATQDPVLRKRFKGTPEH VVNYFFFVAEEVREILASLGARRLDDIIGSSDLLDRDRMIEHWKARGLDFSRIFHKVEAP KEATYWTERQNHPIDDILDRRLIEKAKLALETKVPVAFEAAIKNVDRSAGAMLSGALAKR WGHKGLKDDTIHVTLKGTAGQSFGAFLARGITFDLVGDGNDYVGKGLSGGRIIVRPPENA RIVPHQSIIVGNTVLYGAISGECYFNGVAGERFAVRNSGAIAVVEGVGDHGCEYMTGGVV VVLGGTGRNFAAGMSGGVAYVLDEEGDFARRCNMAMVELQPVPEEDDMLEKLHHHGGDLM HKGMVDVSGDMTRHDEERLYQLISNHLHYTGSVRAKEILDHWADYRPKFRKVMPVEYRRA LEDMERMKMAEAAE