Protein Info for SMc04016 in Sinorhizobium meliloti 1021

Annotation: homospermidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details PF03435: Sacchrp_dh_NADP" amino acids 16 to 160 (145 residues), 120.2 bits, see alignment E=7.5e-39 PF16653: Sacchrp_dh_C" amino acids 164 to 451 (288 residues), 127.5 bits, see alignment E=1e-40

Best Hits

Swiss-Prot: 79% identical to HSS_RHILO: Homospermidine synthase (hss) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K00808, homospermidine synthase [EC: 2.5.1.44] (inferred from 100% identity to smk:Sinme_2855)

MetaCyc: 73% identical to homospermidine synthase monomer (Blastochloris viridis)
Homospermidine synthase. [EC: 2.5.1.44, 2.5.1.45]; 2.5.1.44 [EC: 2.5.1.44, 2.5.1.45]; 2.5.1.44 [EC: 2.5.1.44, 2.5.1.45]

Predicted SEED Role

"Homospermidine synthase (EC 2.5.1.44)" (EC 2.5.1.44)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.44 or 2.5.1.45

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92M54 at UniProt or InterPro

Protein Sequence (483 amino acids)

>SMc04016 homospermidine synthase (Sinorhizobium meliloti 1021)
MADTTYPVYGEITGPIVMIGFGSIGHGTLPLIERHFKYDKNRLIVVEPREDAKDTEIFVR
HGVRHVRAAVTRDNYKELLKPLLTEGGGQGFCVNLSVDTSSLDIIKLCRKLDVLYVDTVI
EPWLGFYFDAEMDNAARTNYALRETVRREKEKNPGGTTAVSTCGANPGMVSWFVKKALLN
LADDLGLKYEEPHQDDREGWAKLMKKAGVKGVHIAERDTQRAKHPKPLNVFWNTWSVEGF
ISEGLQPAELGWGTHENWMPKNAKKHKKGCKAAIYLEQPGANTRVRSWCPTPGPQYGFLV
THNESISIADFFTVRDKDGEVSYRPTCHYAYHPANDAVLSLHEMFGNGGKAQPELHVLDE
HELVDGVDELGVLLYGHAKNAYWYGSRLSLEETRRIAPYQNATGLQVTSAVLAGMVWALE
NPKAGIVEADEMDYKRCLEVQMPYLGPVEGHYTDWTPLDGRPGLFPEDIDTKDPWQFRNI
LVR