Protein Info for SMc03879 in Sinorhizobium meliloti 1021

Updated annotation (from data): Acetyl-CoA acetyltransferase (EC 2.3.1.9)
Rationale: Specifically important for utilizing L-Lysine. Automated validation from mutant phenotype: the predicted function (ACETYL-COA-ACETYLTRANSFER-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: acetyl-CoA acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to THIL_RHIME: Acetyl-CoA acetyltransferase (phaA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00626, acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (inferred from 100% identity to smk:Sinme_3259)

MetaCyc: 77% identical to acetyl-CoA acetyltransferase (Cereibacter sphaeroides)
Acetyl-CoA C-acyltransferase. [EC: 2.3.1.16, 2.3.1.9]

Predicted SEED Role

"Acetyl-CoA acetyltransferase (EC 2.3.1.9)" in subsystem Acetyl-CoA fermentation to Butyrate or Archaeal lipids or Butanol Biosynthesis or Isoprenoid Biosynthesis or MLST or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle (EC 2.3.1.9)

MetaCyc Pathways

oleate β-oxidation (29/35 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (22/26 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (5/5 steps found)
acetyl-CoA fermentation to butanoate (6/7 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
polyhydroxybutanoate biosynthesis (3/3 steps found)
fatty acid salvage (5/6 steps found)
acetoacetate degradation (to acetyl CoA) (2/2 steps found)
glycerol degradation to butanol (12/16 steps found)
(2S)-ethylmalonyl-CoA biosynthesis (3/4 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
ketolysis (2/3 steps found)
L-isoleucine degradation I (4/6 steps found)
pyruvate fermentation to butanol II (engineered) (4/6 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
glutaryl-CoA degradation (3/5 steps found)
ketogenesis (3/5 steps found)
pyruvate fermentation to hexanol (engineered) (7/11 steps found)
2-methyl-branched fatty acid β-oxidation (9/14 steps found)
5,6-dehydrokavain biosynthesis (engineered) (6/10 steps found)
L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
propanoate fermentation to 2-methylbutanoate (3/6 steps found)
superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
valproate β-oxidation (5/9 steps found)
4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
pyruvate fermentation to acetone (2/5 steps found)
pyruvate fermentation to butanol I (4/8 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
pyruvate fermentation to butanoate (3/7 steps found)
4-ethylphenol degradation (anaerobic) (2/6 steps found)
ethylbenzene degradation (anaerobic) (1/5 steps found)
fatty acid β-oxidation VII (yeast peroxisome) (1/5 steps found)
isopropanol biosynthesis (engineered) (1/5 steps found)
2-deoxy-D-ribose degradation II (3/8 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
3-phenylpropanoate degradation (4/10 steps found)
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (4/10 steps found)
10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
2-methylpropene degradation (2/8 steps found)
ethylmalonyl-CoA pathway (4/11 steps found)
mevalonate pathway I (eukaryotes and bacteria) (1/7 steps found)
mevalonate pathway II (haloarchaea) (1/7 steps found)
L-lysine fermentation to acetate and butanoate (3/10 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
4-oxopentanoate degradation (2/9 steps found)
L-glutamate degradation VII (to butanoate) (4/12 steps found)
isoprene biosynthesis II (engineered) (1/8 steps found)
mevalonate pathway III (Thermoplasma) (1/8 steps found)
mevalonate pathway IV (archaea) (1/8 steps found)
methyl tert-butyl ether degradation (2/10 steps found)
10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
L-tryptophan degradation III (eukaryotic) (3/15 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
jasmonic acid biosynthesis (4/19 steps found)
toluene degradation VI (anaerobic) (3/18 steps found)
cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
sitosterol degradation to androstenedione (1/18 steps found)
androstenedione degradation I (aerobic) (6/25 steps found)
platensimycin biosynthesis (6/26 steps found)
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
superpathway of testosterone and androsterone degradation (6/28 steps found)
androstenedione degradation II (anaerobic) (4/27 steps found)
superpathway of ergosterol biosynthesis I (3/26 steps found)
superpathway of L-lysine degradation (15/43 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)
superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
superpathway of cholesterol biosynthesis (3/38 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (8/47 steps found)
superpathway of cholesterol degradation III (oxidase) (4/49 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P50174 at UniProt or InterPro

Protein Sequence (393 amino acids)

>SMc03879 Acetyl-CoA acetyltransferase (EC 2.3.1.9) (Sinorhizobium meliloti 1021)
MSNPSIVIASAARTAVGSFNGAFGNTLAHELGAAAIKAVLERAGVEAGEVDEVILGQVLP
AGEGQNPARQAAMKAGLPQEKTAWGMNQLCGSGLRAVALGMQQIATGDAKVIVAGGMESM
SMAPHCAHLRGGVKMGDYKMIDTMIKDGLTDAFYGYHMGITAENVARKWQLTREEQDEFA
LASQNKAEAAQKAGRFADEIVPFVVKTRKGDVNVDQDEYIRHGATLDSIAKLRPAFDKEG
TVTAGNASGLNDGAAAALLMTEAEAARRGIQPLARIVSWATAGVDPQIMGTGPIPASRKA
LEKAGWSVADIELVEANEAFAAQACAVNKDLGWDPSIVNVNGGAIAIGHPIGASGARVLN
TLLFEMKRRGVSKGLATLCIGGGMGVAMCVERL