Protein Info for SMc02804 in Sinorhizobium meliloti 1021

Annotation: leucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 876 TIGR00396: leucine--tRNA ligase" amino acids 6 to 876 (871 residues), 1009.4 bits, see alignment E=6.7e-308 PF00133: tRNA-synt_1" amino acids 15 to 226 (212 residues), 96.8 bits, see alignment E=2.4e-31 amino acids 431 to 587 (157 residues), 34.9 bits, see alignment E=1.6e-12 amino acids 631 to 671 (41 residues), 32 bits, see alignment 1.2e-11 PF09334: tRNA-synt_1g" amino acids 38 to 172 (135 residues), 73.6 bits, see alignment E=2.7e-24 amino acids 631 to 674 (44 residues), 23.7 bits, see alignment 3.9e-09 PF13603: tRNA-synt_1_2" amino acids 222 to 412 (191 residues), 215.6 bits, see alignment E=8.4e-68 PF08264: Anticodon_1" amino acids 717 to 840 (124 residues), 77.5 bits, see alignment E=2e-25

Best Hits

Swiss-Prot: 82% identical to SYL2_RHIL3: Leucine--tRNA ligase 2 (leuS2) from Rhizobium leguminosarum bv. viciae (strain 3841)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 80% identity to ara:Arad_4935)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92KW8 at UniProt or InterPro

Protein Sequence (876 amino acids)

>SMc02804 leucyl-tRNA synthetase (Sinorhizobium meliloti 1021)
MATERYNPRDAEPRWQHQWEAGKVFETKNDDPRDKYYVLEMFPYPSGRIHMGHVRNYTMG
DVVARYKRARGFNVLHPMGWDAFGMPAENAAMERGVHPASWTYQNIASMKAQLKVMGLSL
DWSREFATCDPEYYQRQQHLFLDFLEKGLVYRKQSKVNWDPVDNTVLANEQVIEGRGWRS
GALVEQRELTQWFFRITDFSQDLLDALDTLDEWPEKVRLMQKNWIGRSEGLSVRWELDPA
TVPGEEKEVTVYTTRPDTLFGASFLAISADHPLARDAAAKNAEIEAFCEECRRAGTSLAA
LETAEKKGIDTGIRARHPFDPNWELPVYVANFVLMDYGTGAIFGCPSGDQRDLDFARKYD
LPVVPVVMPKDADPQTFTIGDEAYDGDGVMINSRFLDGLSAEEAFETVASRLENDLLNGT
PRAERKVNFRLRDWGISRQRYWGCPIPVIHCDDCGVVPVPKADLPVTLPPDVTFDKPGNP
LDRHPTWRHVACPQCGKDARRETDTMDTFVDSSWYFTRFTAPWEDNPTDPKAANHWLPVD
QYIGGIEHAILHLLYSRFFTRAMKATGHVALDEPFKGLFTQGMVVHETYSRGEGAQREWI
TPAEIRVEEVDGQRRAVHIETGEEVAIGSIEKMSKSKKNVVDPDDIIGSYGADTARFFVL
SDSPPDRDVIWSEAGVEGAHRFVQRVWRLLTEAAERLRAVDAAPASGGEGLAVSQAAHRT
LKAVEADYDKLAFNKAVARIYELVNAMAAPLTQVASGKADNALTAAVKDAAAILINLIAP
MMPHLAEECWREIGGKGLIAERPWPKFDPALIVENEITLPVQINGKKRADLTIARDADQS
AIESAVLALDAVKAALNGGRPKKIIVVPQRIVNVVV